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dc.contributor.authorOsorio, Estrella
dc.contributor.authorToledano-Osorio, Manuel
dc.contributor.authorOsorio Ruiz, Raquel 
dc.contributor.authorSánchez Aguilera, Fátima 
dc.contributor.authorMedina-Castillo, Antonio L.
dc.contributor.authorToledano Pérez, Manuel 
dc.identifier.citationOsorio R, Osorio E, Aguilera FS, Medina-Castillo AL, Toledano M, Toledano-Osorio M. Silver improves collagen structure and stability at demineralized dentin: A dynamic-mechanical and Raman analysis. J Dent. 2018 Oct 5. pii: S0300-5712(18)30537-2. []es_ES
dc.description.abstractObjective: This study aimed to evaluate the effect of silver loaded nanoparticles (NPs) application on dentin remineralization. Methods: Polymethylmetacrylate-based NPs and silver loaded NPs (Ag-NPs) were applied on demineralized dentin surfaces. Dentin was characterized morphologically by scanning electron microscopy, mechanically probed by a nanoindenter to test nanohardness and Young modulus, and chemically analyzed by Raman spectroscopy after 24 h and 7 d of storage. Untreated surfaces were used as control. Data were submitted to ANOVA and Student-Newman-Keuls multiple comparisons tests (P<0.05). Results: After Raman analysis, dentin treated with Ag-NPs obtained the lowest mineralization and intensity of stoichiometric hydroxyapatite when compared with specimens treated with undoped-NPs. The lowest relative mineral concentration, expressed as the ratio phosphate or carbonate/phenyl group, and crystallinity was attained by dentin treated with with Ag-NPs, after 7 d. Ag-NPs application generated the highest values of collagen crosslinking (intensity at 1032 cm-1 band). The molecular conformation of the collagen’s polypeptide chains, amide-I and CH2 also attained the highest peaks in dentin treated with Ag-NPs. Staggered and demineralized collagen fibrils were observed covering the dentin surfaces treated with Ag-NPs, at both 24 h and 7 d. Samples treated with Ag-NPs attained the lowest values of nanohardness and Young’s modulus at 7 d of storage. Conclusions: Peritubular and intertubular dentin were remineralized when using undoped-NPs. After 7 d, collagen treated with NPs was remineralized but dentin treated with Ag-NPs attained an improved collagen matrix structure and stability but the lowest mineralization and crystallinity.es_ES
dc.description.sponsorshipThis work was supported by the Ministry of Economy and Competitiveness (MINECO) and European Regional Development Fund (FEDER). Project MAT2017-85999-P MINECO/AEI/FEDER/UE.es_ES
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España*
dc.subjectCollagen es_ES
dc.subjectErosion es_ES
dc.subjectMicroscopy es_ES
dc.titleSilver improves collagen structure and stability at demineralized dentin: a dynamic-mechanical and Raman analysis.es_ES

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Atribución-NoComercial-SinDerivadas 3.0 España
Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 España