Advanced zinc-doped adhesives for high performance at the resin-carious dentin interface
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AuthorToledano Pérez, Manuel; Osorio Ruiz, Raquel; Osorio Ruiz, María Estrella; García-Godoy, Franklin; Toledano Osorio, Manuel; Sánchez Aguilera, Fátima
CariesDentinRemineralizationLoad cyclingZn-doped adhesivesRaman
Toledano Pérez, M.; et al. Advanced zinc-doped adhesives for high performance at the resin-carious dentin interface. Journal of the Mechanical Behavior Biomedical Materials, 62: 247-267 (2016). [http://hdl.handle.net/10481/46728]
SponsorshipProject MAT2014-52036-P supported by the Ministry of Economy and Competitiveness (MINECO) and European Regional Development Fund (FEDER).
The purpose of this study was to evaluate the remineralization ability of an etch-and-rinse Zn-doped resin applied on caries-affected dentin (CAD). CAD surfaces were subjected to: i) 37% phosphoric acid (PA) or ii) 0.5 M ethylenediaminetetraacetic acid (EDTA). 10 wt% ZnO nanoparticles or 2 wt% ZnCl2 were added into the adhesive Single Bond (SB), to create the following groups: PA+SB, PA+SB-ZnO, PA+SB-ZnCl2, EDTA+SB, EDTA+SB-ZnO, EDTA+SB-ZnCl2. Bonded interfaces were submitted to mechanical loading or stored during 24 h. Remineralization of the bonded interfaces was studied by AFM nano-indentation (hardness and Young’s modulus), Raman spectroscopy [mapping with principal component analysis (PCA), and hierarchical cluster analysis (HCA)] and Masson's trichrome staining technique. Dentin samples treated with PA+SB-ZnO attained the highest values of nano-mechanical properties. Load cycling increased both mineralization and crystallographic maturity at the interface; this effect was specially noticed when using ZnCl2-doped resin in EDTA-treated carious dentin. Crosslinking attained higher frequencies indicating better conformation and organization of collagen in specimens treated with PA+SB-ZnO, after load cycling. Trichrome staining technique depicted a deeper demineralized dentin fringe that became reduced after loading, and it was not observable in EDTA+SB groups. Multivariate analysis confirmed de homogenizing effect of load cycling in the percentage of variances, traces of centroids and distribution of clusters, especially in specimens treated with EDTA+SB-ZnCl2.