@misc{10481/46728,
year = {2016},
month = {5},
url = {http://hdl.handle.net/10481/46728},
abstract = {The purpose of this study was to evaluate the remineralization ability of an etch-and-rinse Zn-doped resin applied on caries-affected dentin (CAD). CAD surfaces were subjected to: i) 37% phosphoric acid (PA) or ii) 0.5 M ethylenediaminetetraacetic acid (EDTA). 10 wt% ZnO nanoparticles or 2 wt% ZnCl2 were added into the adhesive Single Bond (SB), to create the following groups: PA+SB, PA+SB-ZnO, PA+SB-ZnCl2, EDTA+SB, EDTA+SB-ZnO, EDTA+SB-ZnCl2. Bonded interfaces were submitted to mechanical loading or stored during 24 h.  Remineralization of the bonded interfaces was studied by AFM nano-indentation (hardness and Young’s modulus), Raman spectroscopy [mapping with principal component analysis (PCA), and hierarchical cluster analysis (HCA)] and Masson's trichrome staining technique. Dentin samples treated with PA+SB-ZnO attained the highest values of nano-mechanical properties. Load cycling increased both mineralization and crystallographic maturity at the interface; this effect was specially noticed when using ZnCl2-doped resin in EDTA-treated carious dentin. Crosslinking attained higher frequencies indicating better conformation and organization of collagen in specimens treated with PA+SB-ZnO, after load cycling. Trichrome staining technique depicted a deeper demineralized dentin fringe that became reduced after loading, and it was not observable in EDTA+SB groups. Multivariate analysis confirmed de homogenizing effect of load cycling in the percentage of variances, traces of centroids and distribution of clusters, especially in specimens treated with  EDTA+SB-ZnCl2.},
organization = {Project MAT2014-52036-P supported by the Ministry of Economy and Competitiveness
(MINECO) and European Regional Development Fund (FEDER).},
publisher = {Elsevier},
keywords = {Caries},
keywords = {Dentin},
keywords = {Remineralization},
keywords = {Load cycling},
keywords = {Zn-doped adhesives},
keywords = {Raman},
title = {Advanced zinc-doped adhesives for high performance at the resin-carious dentin interface},
doi = {10.1016/j.jmbbm.2016.05.013},
author = {Toledano Pérez, Manuel and Osorio Ruiz, Raquel and Osorio Ruiz, María Estrella and García-Godoy, Franklin and Toledano Osorio, Manuel and Sánchez Aguilera, Fátima},
}