Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling
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Toledano Pérez, Manuel; Cabello Malagón, Inmaculada; Sánchez Aguilera, Fátima; Osorio Ruiz, María Estrella; Toledano Osorio, Manuel; Osorio Ruiz, RaquelEditorial
Microscopy Society of America
Materia
Dentin Fluoresceine Rodhamine Xylenol orange Confocal microscopy
Date
2015Referencia bibliográfica
Toledano Pérez, M.; et al. Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling. Microscopy and Microanalysis: online (2015). [http://hdl.handle.net/10481/39009]
Sponsorship
This work was supported by grants MINECO/FEDER MAT2011-24551, MAT2014-52036-P, and CEI-Biotic UGR.Abstract
Introduction. The purpose of this study was to investigate the micro-morphology of the
resin-dentin inter-diffusion zone using two different single-bottle self-etching dentin
adhesives with and without previous acid-etching, after in vitro mechanical loading stimuli.
Materials and Methods. Extracted human third molars were sectioned to obtain dentin
surfaces. Two different single-bottle self-etching dentin adhesives, Futurabond U (FUT)
and Experimental (EXP) both from VOCO, were applied following the manufacturer's
instructions or after 37% phosphoric acid application. Resin-dentin interfaces were
analyzed with dye assisted confocal microscopy evaluation (CLSM), including the
calcium-chelation technique, xylenol orange (CLSM-XO). Results. The confocal
microscopy revealed that resin-dentin interfaces of unloaded specimens were deficiently
resin-hybridized, in general. These samples showed a rodhamine B-labeled hybrid complex
and adhesive layer completely affected by fluorescein penetration (nanoleakage) through
the porous resin-dentin interface, but thicker after phosphoric acid-etching. Load cycling
promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the
hybrid complex porosity, and an increment of dentin mineralization. Load cycled
specimens treated with the xylenol orange technique produced a clearly outlined
fluorescence due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when the experimental adhesive was applied.