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Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling
dc.contributor.author | Toledano Pérez, Manuel | |
dc.contributor.author | Cabello Malagón, Inmaculada | |
dc.contributor.author | Sánchez Aguilera, Fátima | |
dc.contributor.author | Osorio Ruiz, María Estrella | |
dc.contributor.author | Toledano Osorio, Manuel | |
dc.contributor.author | Osorio Ruiz, Raquel | |
dc.date.accessioned | 2015-11-26T13:06:58Z | |
dc.date.available | 2015-11-26T13:06:58Z | |
dc.date.issued | 2015 | |
dc.identifier.citation | Toledano Pérez, M.; et al. Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling. Microscopy and Microanalysis: online (2015). [http://hdl.handle.net/10481/39009] | es_ES |
dc.identifier.issn | 1431-9276 | |
dc.identifier.issn | 1154-2799 | |
dc.identifier.uri | http://hdl.handle.net/10481/39009 | |
dc.description.abstract | Introduction. The purpose of this study was to investigate the micro-morphology of the resin-dentin inter-diffusion zone using two different single-bottle self-etching dentin adhesives with and without previous acid-etching, after in vitro mechanical loading stimuli. Materials and Methods. Extracted human third molars were sectioned to obtain dentin surfaces. Two different single-bottle self-etching dentin adhesives, Futurabond U (FUT) and Experimental (EXP) both from VOCO, were applied following the manufacturer's instructions or after 37% phosphoric acid application. Resin-dentin interfaces were analyzed with dye assisted confocal microscopy evaluation (CLSM), including the calcium-chelation technique, xylenol orange (CLSM-XO). Results. The confocal microscopy revealed that resin-dentin interfaces of unloaded specimens were deficiently resin-hybridized, in general. These samples showed a rodhamine B-labeled hybrid complex and adhesive layer completely affected by fluorescein penetration (nanoleakage) through the porous resin-dentin interface, but thicker after phosphoric acid-etching. Load cycling promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the hybrid complex porosity, and an increment of dentin mineralization. Load cycled specimens treated with the xylenol orange technique produced a clearly outlined fluorescence due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when the experimental adhesive was applied. | es_ES |
dc.description.sponsorship | This work was supported by grants MINECO/FEDER MAT2011-24551, MAT2014-52036-P, and CEI-Biotic UGR. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Microscopy Society of America | es_ES |
dc.rights | Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/ | |
dc.subject | Dentin | es_ES |
dc.subject | Fluoresceine | es_ES |
dc.subject | Rodhamine | es_ES |
dc.subject | Xylenol orange | es_ES |
dc.subject | Confocal microscopy | es_ES |
dc.title | Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling | es_ES |
dc.type | preprint | es_ES |
dc.rights.accessRights | open access | es_ES |
dc.identifier.doi | 10.1017/S1431927615015317 |