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dc.contributor.authorFernández-Arroyo, Salvador
dc.contributor.authorHuete-Toral, Fernando
dc.contributor.authorPérez de Lara, María Jesús
dc.contributor.authorCádiz Gurrea, María de la Luz 
dc.contributor.authorLegeai-Mallet, Laurence
dc.contributor.authorMicol Molina, Vicente
dc.contributor.authorSegura Carretero, Antonio 
dc.contributor.authorJoven, Jorge
dc.contributor.authorPintor, Jesús
dc.date.accessioned2015-10-23T07:07:24Z
dc.date.available2015-10-23T07:07:24Z
dc.date.issued2015
dc.identifier.citationFernández-Arroyo, S.; et al. The impact of polyphenols on chondrocyte growth and survival: a preliminary report. Food and Nutrition Research, 59: 29311 (2015). [http://hdl.handle.net/10481/38574]es_ES
dc.identifier.issn1654-6628
dc.identifier.issn1654-661X
dc.identifier.urihttp://hdl.handle.net/10481/38574
dc.description.abstractBackground: Imbalances in the functional binding of fibroblast growth factors (FGFs) to their receptors (FGFRs) have consequences for cell proliferation and differentiation that in chondrocytes may lead to degraded cartilage. The toxic, proinflammatory, and oxidative response of cytokines and FGFs can be mitigated by dietary polyphenols. Objective: We explored the possible effects of polyphenols in the management of osteoarticular diseases using a model based on the transduction of a mutated human FGFR3 (G380R) in murine chondrocytes. This mutation is present in most cases of skeletal dysplasia and is responsible for the overexpression of FGFR3 that, in the presence of its ligand, FGF9, results in toxic effects leading to altered cellular growth. Design: Different combinations of dietary polyphenols derived from plant extracts were assayed in FGFR3 (G380R) mutated murine chondrocytes, exploring cell survival, chloride efflux, extracellular matrix (ECM) generation, and grade of activation of mitogen-activated protein kinases. Results: Bioactive compounds from Hibiscus sabdariffa reversed the toxic effects of FGF9 and restored normal growth, suggesting a probable translation to clinical requests in humans. Indeed, these compounds activated the intracellular chloride efflux, increased ECM generation, and stimulated cell proliferation. The inhibition of mitogen-activated protein kinase phosphorylation was interpreted as the main mechanism governing these beneficial effects. Conclusions: These findings support the rationale behind the encouragement of the development of drugs that repress the overexpression of FGFRs and suggest the dietary incorporation of supplementary nutrients in the management of degraded cartilage.es_ES
dc.description.sponsorshipThe authors are grateful for the constant support provided by the Hospital Universitari de Sant Joan and the Universitat Rovira i Virgili. Salvador Fernández-Arroyo is the recipient of a Sara Borrell grant (CD12/00672) from the Instituto de Salud Carlos III, Madrid, Spain. The authors also thank the Andalusian Regional Government Council of Innovation and Science for the Excellence Project P11-CTS-7625 and Generalitat Valenciana for the project PROMETEO/2012/007. This work was also supported by projects of the Fundación Areces and the Fundación MAGAR.es_ES
dc.language.isoenges_ES
dc.publisherCo-Action Publishinges_ES
dc.subjectChondrodysplasiaes_ES
dc.subjectFibroblast growth factores_ES
dc.subjectHibiscus sabdariffaes_ES
dc.subjectNutrition es_ES
dc.subjectOsteoarthritis es_ES
dc.subjectPolyphenolses_ES
dc.subjectSignaling pathwayses_ES
dc.titleThe impact of polyphenols on chondrocyte growth and survival: a preliminary reportes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses_ES
dc.identifier.doi10.3402/fnr.v59.29311


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