Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells
Metadatos
Mostrar el registro completo del ítemAutor
Berdasco, María; Alcázar, Rubén; García-Ortiz, María Victoria; Ballestar, Esteban; Fernández, Agustín F.; Roldán-Arjona, Teresa; Tiburcio, Antonio F.; Altabella, Teresa; Buisine, Nicolas; Quesneville, Hadi; Baudry, Antoine; Lepiniec, Loïc; Alaminos Mingorance, Miguel; Rodríguez, Roberto; Lloyd, Alan; Colot, Vicent; Bender, Judith; Canal, María Jesús; Esteller, Manel; Fraga, Mario F.Editorial
Public Library of Science (PLOS)
Materia
Arabidopsis thaliana Cell differentiation DNA Epigenetics Transposable elements
Fecha
2008Referencia bibliográfica
Berdasco, M.; et al. Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells. Plos One, 3(10): e3306 (2008). [http://hdl.handle.net/10481/30773]
Patrocinador
This work was supported by the Health (FIS01-04) (PI061267), Education and Science (I+D+I MCYT08-03, FU2004-02073/BMC and Consolider MEC09-05) Departments of the Spanish Government, the European Grant TRANSFOG LSHC-CT-2004-503438, and the Spanish Association Against Cancer (AECC). M.B. is funded by the Association Against Cancer (AECC).Resumen
Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.