@misc{10481/30773,
year = {2008},
url = {http://hdl.handle.net/10481/30773},
abstract = {Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.},
organization = {This work was supported by the Health (FIS01-04) (PI061267), Education and Science (I+D+I MCYT08-03, FU2004-02073/BMC and Consolider MEC09-05) Departments of the Spanish Government, the European Grant TRANSFOG LSHC-CT-2004-503438, and the Spanish Association Against Cancer (AECC). M.B. is funded by the Association Against Cancer (AECC).},
publisher = {Public Library of Science (PLOS)},
keywords = {Arabidopsis thaliana},
keywords = {Cell differentiation},
keywords = {DNA},
keywords = {Epigenetics},
keywords = {Transposable elements},
title = {Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells},
author = {Berdasco, María and Alcázar, Rubén and García-Ortiz, María Victoria and Ballestar, Esteban and Fernández, Agustín F. and Roldán-Arjona, Teresa and Tiburcio, Antonio F. and Altabella, Teresa and Buisine, Nicolas and Quesneville, Hadi and Baudry, Antoine and Lepiniec, Loïc and Alaminos Mingorance, Miguel and Rodríguez, Roberto and Lloyd, Alan and Colot, Vicent and Bender, Judith and Canal, María Jesús and Esteller, Manel and Fraga, Mario F.},
}