Reverse transcriptase-like activity in Trypanosoma cruzi

dc.contributor.author	González, Clara
dc.contributor.author	thomas, Maria del Carmen
dc.contributor.author	Martín Molina, Francisco
dc.contributor.author	Alcami, j
dc.contributor.author	Alonso, Carlos
dc.contributor.author	Lopez, Manuel Carlos
dc.date.accessioned	2025-01-31T08:13:44Z
dc.date.available	2025-01-31T08:13:44Z
dc.date.issued	1997
dc.identifier.citation	Acta tropica: Vol 63 Páginas 117-126 (1997)	es_ES
dc.identifier.uri	https://hdl.handle.net/10481/101461
dc.description.abstract	The use of specific synthetic RNA homopolymers as templates and short oligonucleotides as primers has allowed evidence of the existence of a reverse transcriptase-like activity in Trypanosoma cruzi, to be revealed. The RNA:DNA products derived from this reaction are of approximately 110 nucleotides in length. The enzyme has greater affinity for poly(rA)/oligo(dT) templates than for poly(rC)/oligo(dG) having a 20 mM Mg++ ion requirement. The detected reverse transcriptase-like activity is not affected by aphidicolin and ddTTP but is inhibited by actinomycin D, novobiocin, rifamycin SV and AZT.	es_ES
dc.language.iso	eng	es_ES
dc.publisher	ScienceDirect - Elsevier	es_ES
dc.subject	Nuclease activity	es_ES
dc.subject	Trypanosoma cruzi	es_ES
dc.subject	LINEs	es_ES
dc.title	Reverse transcriptase-like activity in Trypanosoma cruzi	es_ES
dc.type	journal article	es_ES
dc.rights.accessRights	open access	es_ES
dc.identifier.doi	10.1016/s0001-706x(96)00611-0