Universidad de Granada Digibug
 

Repositorio Institucional de la Universidad de Granada >
1.-Investigación >
Departamentos, Grupos de Investigación e Institutos >
Departamento de Química Física >
DQF - Artículos >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/49157

Title: A Red-Emitting, Multidimensional Sensor for the Simultaneous Cellular Imaging of Biothiols and Phosphate Ions
Authors: Herrero-Foncubierta, Pilar
Paredes Martínez, José Manuel
Girón González, María Dolores
Salto González, Rafael
Cuerva Carvajal, Juan Manuel
Miguel Álvarez, Delia
Orte, Ángel
Issue Date: 9-Jan-2018
Abstract: The development of new fluorescent probes for cellular imaging is currently a very active field because of the large potential in understanding cell physiology, especially targeting anomalous behaviours due to disease. In particular, red-emitting dyes are keenly sought, as the light in this spectral region presents lower interferences and a deeper depth of penetration in tissues. In this work, we have synthesized a red-emitting, dual probe for the multiplexed intracellular detection of biothiols and phosphate ions. We have prepared a fluorogenic construct involving a silicon-substituted fluorescein for red emission. The fluorogenic reaction is selectively started by the presence of biothiols. In addition, the released fluorescent moiety undergoes an excited-state proton transfer reaction promoted by the presence of phosphate ions, which modulates its fluorescence lifetime, τ, with the total phosphate concentration. Therefore, in a multidimensional approach, the intracellular levels of biothiols and phosphate can be detected simultaneously using a single fluorophore and with spectral clearing of cell autofluorescence interferences. We have applied this concept to different cell lines, including photoreceptor cells, whose levels of biothiols are importantly altered by light irradiation and other oxidants.
Sponsorship: This work was funded by grants CTQ2014-56370-R, CTQ2014-53598, and CTQ2014-55474-C2-2-R from the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (ERDF), and grant FQM2012-790 from the Consejería de Innovación, Ciencia y Empresa (Junta de Andalucı́a), including costs to publish in open access.
Publisher: MDPI
Keywords: Dual probes
Fluorescent sensors
Fluorescence lifetime imaging
FLIM
Cellular stress
Photoreceptor cells
URI: http://hdl.handle.net/10481/49157
ISSN: 1424-8220
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Herrero-Foncubierta, P.; et al. A Red-Emitting, Multidimensional Sensor for the Simultaneous Cellular Imaging of Biothiols and Phosphate Ions. Sensors, 18(1): 161 (2018). [http://hdl.handle.net/10481/49157]
Appears in Collections:DQF - Artículos

Files in This Item:

File Description SizeFormat
HerreroFoncubierta_FluorescentSensors.pdf5.11 MBAdobe PDFView/Open
Recommend this item

This item is licensed under a Creative Commons License
Creative Commons

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! OpenAire compliant DSpace Software Copyright © 2002-2007 MIT and Hewlett-Packard - Feedback

© Universidad de Granada