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dc.contributor.authorHerrero-Foncubierta, Pilares_ES
dc.contributor.authorParedes Martínez, José Manuel es_ES
dc.contributor.authorGirón González, María Dolores es_ES
dc.contributor.authorSalto González, Rafael es_ES
dc.contributor.authorCuerva Carvajal, Juan Manuel es_ES
dc.contributor.authorMiguel Álvarez, Delia es_ES
dc.contributor.authorOrte Gutiérrez, Ángel es_ES
dc.date.accessioned2018-01-24T13:47:44Z
dc.date.available2018-01-24T13:47:44Z
dc.date.issued2018-01-09
dc.identifier.citationHerrero-Foncubierta, P.; et al. A Red-Emitting, Multidimensional Sensor for the Simultaneous Cellular Imaging of Biothiols and Phosphate Ions. Sensors, 18(1): 161 (2018). [http://hdl.handle.net/10481/49157]es_ES
dc.identifier.issn1424-8220
dc.identifier.urihttp://hdl.handle.net/10481/49157
dc.description.abstractThe development of new fluorescent probes for cellular imaging is currently a very active field because of the large potential in understanding cell physiology, especially targeting anomalous behaviours due to disease. In particular, red-emitting dyes are keenly sought, as the light in this spectral region presents lower interferences and a deeper depth of penetration in tissues. In this work, we have synthesized a red-emitting, dual probe for the multiplexed intracellular detection of biothiols and phosphate ions. We have prepared a fluorogenic construct involving a silicon-substituted fluorescein for red emission. The fluorogenic reaction is selectively started by the presence of biothiols. In addition, the released fluorescent moiety undergoes an excited-state proton transfer reaction promoted by the presence of phosphate ions, which modulates its fluorescence lifetime, τ, with the total phosphate concentration. Therefore, in a multidimensional approach, the intracellular levels of biothiols and phosphate can be detected simultaneously using a single fluorophore and with spectral clearing of cell autofluorescence interferences. We have applied this concept to different cell lines, including photoreceptor cells, whose levels of biothiols are importantly altered by light irradiation and other oxidants.en_EN
dc.description.sponsorshipThis work was funded by grants CTQ2014-56370-R, CTQ2014-53598, and CTQ2014-55474-C2-2-R from the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (ERDF), and grant FQM2012-790 from the Consejería de Innovación, Ciencia y Empresa (Junta de Andalucı́a), including costs to publish in open access.en_EN
dc.language.isoenges_ES
dc.publisherMDPIes_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es_ES
dc.subjectDual probesen_EN
dc.subjectFluorescent sensorsen_EN
dc.subjectFluorescence lifetime imagingen_EN
dc.subjectFLIMen_EN
dc.subjectCellular stressen_EN
dc.subjectPhotoreceptor cellsen_EN
dc.titleA Red-Emitting, Multidimensional Sensor for the Simultaneous Cellular Imaging of Biothiols and Phosphate Ionsen_EN
dc.typeinfo:eu-repo/semantics/articleen_EN
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen_EN
dc.identifier.doi10.3390/s18010161


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