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dc.contributor.authorReyes Zurita, Fernando Jesús 
dc.contributor.authorRufino Palomares, Eva Encarnación
dc.contributor.authorGarcía-Salguero, Leticia
dc.contributor.authorPeragón Sánchez, Juan
dc.contributor.authorMedina, Pedro P.
dc.contributor.authorParra Sánchez, Andrés 
dc.contributor.authorCascante Serratosa, Marta
dc.contributor.authorLupiáñez Cara, José Antonio
dc.identifier.citationReyes Zurita, F.J.; et al. Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells. Plos One, 11(1): e0146178 (2016). []es_ES
dc.description.abstractMaslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dose-dependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases -8 and -3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.es_ES
dc.description.sponsorshipThis study was supported by grants Group BIO 157 from the Technology and Innovation Council of the Andalucian regional government and AGL2006-12210-C03-02/ALI, SAF2005-01627, ISCIII-RTICC (RD06/0020/0046) from the Spanish government and European Union FEDER funds.es_ES
dc.publisherPublic Library of Science (PLOS)es_ES
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivs 3.0 Licensees_ES
dc.subjectCaco-2 cellses_ES
dc.subjectHT29 cellses_ES
dc.subjectProtein expressiones_ES
dc.subjectCell cycle and cell divisiones_ES
dc.subjectCell differentiationes_ES
dc.subjectApoptotic signaling cascadees_ES
dc.titleMaslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cellses_ES

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