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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/39583

Title: Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells
Authors: Reyes Zurita, Fernando Jesús
Rufino Palomares, Eva Encarnación
García-Salguero, Leticia
Peragón Sánchez, Juan
Medina, Pedro P.
Parra Sánchez, Andrés
Cascante Serratosa, Marta
Lupiáñez Cara, José Antonio
Issue Date: 2016
Abstract: Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mitochondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dose-dependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases -8 and -3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondrial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin.
Sponsorship: This study was supported by grants Group BIO 157 from the Technology and Innovation Council of the Andalucian regional government and AGL2006-12210-C03-02/ALI, SAF2005-01627, ISCIII-RTICC (RD06/0020/0046) from the Spanish government and European Union FEDER funds.
Publisher: Public Library of Science (PLOS)
Keywords: Apoptosis
Caco-2 cells
HT29 cells
Mitochondria
Protein expression
Cell cycle and cell division
Cell differentiation
Apoptotic signaling cascade
URI: http://hdl.handle.net/10481/39583
ISSN: 1932-6203
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Reyes Zurita, F.J.; et al. Maslinic Acid, a Natural Triterpene, Induces a Death Receptor-Mediated Apoptotic Mechanism in Caco-2 p53-Deficient Colon Adenocarcinoma Cells. Plos One, 11(1): e0146178 (2016). [http://hdl.handle.net/10481/39583]
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