Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling
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AutorToledano Pérez, Manuel; Cabello Malagón, Inmaculada; Aguilera, Fátima; Osorio Ruiz, Estrella; Toledano-Osorio, Manuel; Osorio Ruiz, Raquel
Microscopy Society of America
DentinFluoresceineRodhamineXylenol orangeConfocal microscopy
Toledano Pérez, M.; et al. Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling. Microscopy and Microanalysis: online (2015). [http://hdl.handle.net/10481/39009]
PatrocinadorThis work was supported by grants MINECO/FEDER MAT2011-24551, MAT2014-52036-P, and CEI-Biotic UGR.
Introduction. The purpose of this study was to investigate the micro-morphology of the resin-dentin inter-diffusion zone using two different single-bottle self-etching dentin adhesives with and without previous acid-etching, after in vitro mechanical loading stimuli. Materials and Methods. Extracted human third molars were sectioned to obtain dentin surfaces. Two different single-bottle self-etching dentin adhesives, Futurabond U (FUT) and Experimental (EXP) both from VOCO, were applied following the manufacturer's instructions or after 37% phosphoric acid application. Resin-dentin interfaces were analyzed with dye assisted confocal microscopy evaluation (CLSM), including the calcium-chelation technique, xylenol orange (CLSM-XO). Results. The confocal microscopy revealed that resin-dentin interfaces of unloaded specimens were deficiently resin-hybridized, in general. These samples showed a rodhamine B-labeled hybrid complex and adhesive layer completely affected by fluorescein penetration (nanoleakage) through the porous resin-dentin interface, but thicker after phosphoric acid-etching. Load cycling promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the hybrid complex porosity, and an increment of dentin mineralization. Load cycled specimens treated with the xylenol orange technique produced a clearly outlined fluorescence due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when the experimental adhesive was applied.