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Please use this identifier to cite or link to this item: http://hdl.handle.net/10481/39009

Title: Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling
Authors: Toledano Pérez, Manuel
Cabello Malagón, Inmaculada
Aguilera, Fátima
Osorio Ruiz, Estrella
Toledano-Osorio, Manuel
Osorio Ruiz, Raquel
Issue Date: 2015
Abstract: Introduction. The purpose of this study was to investigate the micro-morphology of the resin-dentin inter-diffusion zone using two different single-bottle self-etching dentin adhesives with and without previous acid-etching, after in vitro mechanical loading stimuli. Materials and Methods. Extracted human third molars were sectioned to obtain dentin surfaces. Two different single-bottle self-etching dentin adhesives, Futurabond U (FUT) and Experimental (EXP) both from VOCO, were applied following the manufacturer's instructions or after 37% phosphoric acid application. Resin-dentin interfaces were analyzed with dye assisted confocal microscopy evaluation (CLSM), including the calcium-chelation technique, xylenol orange (CLSM-XO). Results. The confocal microscopy revealed that resin-dentin interfaces of unloaded specimens were deficiently resin-hybridized, in general. These samples showed a rodhamine B-labeled hybrid complex and adhesive layer completely affected by fluorescein penetration (nanoleakage) through the porous resin-dentin interface, but thicker after phosphoric acid-etching. Load cycling promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the hybrid complex porosity, and an increment of dentin mineralization. Load cycled specimens treated with the xylenol orange technique produced a clearly outlined fluorescence due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when the experimental adhesive was applied.
Sponsorship: This work was supported by grants MINECO/FEDER MAT2011-24551, MAT2014-52036-P, and CEI-Biotic UGR.
Publisher: Microscopy Society of America
Keywords: Dentin
Fluoresceine
Rodhamine
Xylenol orange
Confocal microscopy
URI: http://hdl.handle.net/10481/39009
ISSN: 1431-9276
1154-2799
Rights : Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License
Citation: Toledano Pérez, M.; et al. Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling. Microscopy and Microanalysis: online (2015). [http://hdl.handle.net/10481/39009]
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