Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling Toledano Pérez, Manuel Cabello Malagón, Inmaculada Sánchez Aguilera, Fátima Osorio Ruiz, María Estrella Toledano Osorio, Manuel Osorio Ruiz, Raquel Dentin Fluoresceine Rodhamine Xylenol orange Confocal microscopy Introduction. The purpose of this study was to investigate the micro-morphology of the resin-dentin inter-diffusion zone using two different single-bottle self-etching dentin adhesives with and without previous acid-etching, after in vitro mechanical loading stimuli. Materials and Methods. Extracted human third molars were sectioned to obtain dentin surfaces. Two different single-bottle self-etching dentin adhesives, Futurabond U (FUT) and Experimental (EXP) both from VOCO, were applied following the manufacturer's instructions or after 37% phosphoric acid application. Resin-dentin interfaces were analyzed with dye assisted confocal microscopy evaluation (CLSM), including the calcium-chelation technique, xylenol orange (CLSM-XO). Results. The confocal microscopy revealed that resin-dentin interfaces of unloaded specimens were deficiently resin-hybridized, in general. These samples showed a rodhamine B-labeled hybrid complex and adhesive layer completely affected by fluorescein penetration (nanoleakage) through the porous resin-dentin interface, but thicker after phosphoric acid-etching. Load cycling promoted an improved sealing of the resin-dentin interface at dentin, a decrease of the hybrid complex porosity, and an increment of dentin mineralization. Load cycled specimens treated with the xylenol orange technique produced a clearly outlined fluorescence due to a consistent Ca-mineral deposits within the bonding interface and inside the dentinal tubules, especially when the experimental adhesive was applied. 2015-11-26T13:06:58Z 2015-11-26T13:06:58Z 2015 info:eu-repo/semantics/preprint Toledano Pérez, M.; et al. Improved sealing and remineralization at the resin-dentin interface after phosphoric acid etching and load cycling. Microscopy and Microanalysis: online (2015). [http://hdl.handle.net/10481/39009] 1431-9276 1154-2799 http://hdl.handle.net/10481/39009 10.1017/S1431927615015317 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Microscopy Society of America