Gut microbiota contributes to the development of hypertension in a genetic mouse model of systemic lupus erythematosus Visitación Pastor, Néstor de la Robles Vera, Iñaki Toral Jiménez, Marta Gómez Guzmán, Manuel Sánchez Santos, Manuel Moleón Moya, Javier González Correa, Cristina Martín Morales, Natividad O'Valle Ravassa, Francisco Javier Jiménez Moleón, Rosario Romero Pérez, Miguel Duarte Pérez, Juan Manuel Endothelial dysfunction Gut dysbiosis Hypertension https://pubmed.ncbi.nlm.nih.gov/33931880/ https://bpspubs.onlinelibrary.wiley.com/doi/10.1111/bph.15512 https://bpspubs.onlinelibrary.wiley.com/doi/epdf/10.1111/bph.15512 Hypertension is an important cardiovascular risk factor that is prevalent in the systemic lupus erythematosus patient population. Here, we have investigated whether intestinal microbiota is involved in hypertension in a genetic mouse model of systemic lupus erythematosus. Twenty-six-week-old female NZW/LacJ (control) and NZBWF1 (F1 hybrid of New Zealand Black and New Zealand White strains; systemic lupus erythematosus) mice were treated for 6 weeks with a broad-spectrum antibiotic mixture or with vancomycin. Faecal microbiota transplantation was performed from donor systemic lupus erythematosus group to recipient to germ-depleted or germ-free mice. Antibiotic treatment inhibited the development of hypertension and renal injury, improved endothelial dysfunction and vascular oxidative stress, and decreased aortic Th17 infiltration in NZBWF1 mice. High BP and vascular complications found in systemic lupus erythematosus mice, but not autoimmunity, kidney inflammation and endotoxemia, were reproduced by the transfer of gut microbiota from systemic lupus erythematosus donors to germ-free or germ-depleted mice. Increased proportions of Bacteroides were linked with high BP in these mice. The reduced endothelium-dependent vasodilator responses to acetylcholine and the high BP induced by microbiota from hypertensive systemic lupus erythematosus mice were inhibited after IL-17 neutralization. Changes in T-cell populations, endothelial function, vascular inflammation and hypertension driven by a genetic systemic lupus erythematosus background can be modified by antibiotic-induced changes in gut microbiota. The vascular changes induced by hypertensive systemic lupus erythematosus microbiota were mediated by Th17 infiltration in the vasculature. 2024-01-29T11:52:17Z 2024-01-29T11:52:17Z 2021-09 info:eu-repo/semantics/article de la Visitación N, Robles-Vera I, Toral M, Gómez-Guzmán M, Sánchez M, Moleón J, González-Correa C, Martín-Morales N, O'Valle F, Jiménez R, Romero M, Duarte J. Gut microbiota contributes to the development of hypertension in a genetic mouse model of systemic lupus erythematosus. Br J Pharmacol. 2021 Sep;178(18):3708-3729. doi: 10.1111/bph.15512. Epub 2021 Jun 16. PMID: 33931880. 0007-1188 PMID: 33931880 https://hdl.handle.net/10481/87491 10.1111/bph.15512 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Wiley