Exchangeable Self-Assembled Lanthanide Antennas for PLIM Microscopy Ruiz Arias, Álvaro Fueyo-Gonzalez, Francisco Izquierdo-Garcia, Carolina Navarro, Amparo Gutierrez-Rodriguez, Marta Herranz, Rosario Burgio, Chiara Reinoso, Antonio Cuerva Carvajal, Juan Manuel Orte Gutiérrez, Ángel González Vera, Juan Antonio This is the published manuscript version of the following manuscript: Exchangeable Self-Assembled Lanthanide Antennas for PLIM Microscopy, Angew. Chem. Int. Ed. 2023, e202314595 doi: https://doi.org/10.1002/anie.202314595 This article may be used for non-commercial purposes in accordance with Wiley Sharing Policies. Supplementary Materials accompanying this article can be found on-line at the publisher’s site. Lanthanides have unique photoluminescence (PL) emission properties, including very long PL life- times. This makes them ideal for biological imaging applications, especially using PL lifetime imaging micro- scopy (PLIM). PLIM is an inherently multidimensional technique with exceptional advantages for quantitative biological imaging. Unfortunately, due to the required prolonged acquisitions times, photobleaching of lantha- nide PL emission currently constitutes one of the main drawbacks of PLIM. In this study, we report a small aqueous-soluble, lanthanide antenna, 8-methoxy-2-oxo- 1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-phosphonic acid, PAnt, specifically designed to dynamically interact with lanthanide ions, serving as exchangeable dye aimed at mitigating photobleaching in PLIM microscopy in cellulo. Thus, self-assembled lanthanide complexes that may be photobleached during image acquisition are continuously replenished by intact lanthanide antennas from a large reservoir. Remarkably, our self-assembled lanthanide complex clearly demonstrated a significant reduction of PL photobleaching when compared to well- established lanthanide cryptates, used for bioimaging. This concept of exchangeable lanthanide antennas opens new possibilities for quantitative PLIM bioimaging. 2023-12-04T07:39:44Z 2023-12-04T07:39:44Z 2023 info:eu-repo/semantics/article Angew. Chem. Int. Ed. 2023, e202314595 doi.org/10.1002/anie.202314595 https://hdl.handle.net/10481/86002 10.1002/anie.202314595 eng http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional Wiley