Estrone, the major postmenopausal estrogen, binds ERa to induce SNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancer metastasis Qureshi, Rehana Picón Ruiz, Manuel Díaz Ruano, Ana Belén Recent work showed that the dominant post-menopausal estrogen, estrone, cooperates with nuclear factor kB (NF-kB) to stimulate inflammation, while pre-menopausal 17b-estradiol opposes NF-kB. Here, we show that post-menopausal estrone, but not 17b-estradiol, activates epithelial-to-mesenchymal transition (EMT) genes to stimulate breast cancer metastasis. HSD17B14, which converts 17b-estradiol to estrone, is higher in cancer than normal breast tissue and in metastatic than primary cancers and associates with earlier metastasis. Treatment with estrone, but not 17b-estradiol, and HSD17B14 overexpression both stimulate an EMT, matrigel invasion, and lung, bone, and liver metastasis in estrogen-receptor-positive (ER+) breast cancer models, while HSD17B14 knockdown reverses the EMT. Estrone:ERa recruits CBP/p300 to the SNAI2 promoter to induce SNAI2 and stimulate an EMT, while 17b-estradiol:ERa recruits co-repressors HDAC1 and NCOR1 to this site. Present work reveals novel differences in gene regulation by these estrogens and the importance of estrone to ER+ breast cancer progression. Upon loss of 17b-estradiol at menopause, estrone-liganded ERa would promote ER+ breast cancer invasion and metastasis. 2023-01-12T13:25:49Z 2023-01-12T13:25:49Z 2022-11-15 info:eu-repo/semantics/article Rehana Qureshi... [et al.]. Estrone, the major postmenopausal estrogen, binds ERa to induce SNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancer metastasis, Cell Reports, Volume 41, Issue 7, 2022, 111672, ISSN 2211-1247, [https://doi.org/10.1016/j.celrep.2022.111672] https://hdl.handle.net/10481/78958 10.1016/j.celrep.2022.111672 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Atribución 4.0 Internacional Cell Press