Methodological procedures explain observed differences in the competence of European populations of Aedes albopictus for the transmission of Zika virus Gutiérrez-López, Rafael Figuerola, Jordi Martínez-de la Puentede, Josué Zika virus Mosquitos The risk of autochthonous transmission of Zika virus (ZIKV) in Europe greatly depends on the capacity of Aedes albopictus to transmit ZIKV. Experimental studies of the vector competence of European populations of this invasive mosquito species provided contrasting results, that may suggest differences between mosquito populations in ZIKV competence and/or differences in the methodological procedures used on the experimental studies. We systematically reviewed published studies of experimental mosquito infections and show that differences detected in the infection and transmission rates of Ae. albopictus are mostly due to differences between studies in the temperature, the viral load used to inoculate mosquitoes, and the number of days post-exposure (dpe) to the virus at which mosquitoes were analysed. In particular, the proportion of infected mosquitoes was affected by the viral load inoculated and the dpe, whereas the transmission rate was influenced by the viral load and temperature. Thus, the methodological differences used in these studies greatly affect the conclusions obtained regarding the role of Ae. albopictus in Europe as a competent vector for ZIKV and its epidemiological relevance. 2022-10-28T10:03:03Z 2022-10-28T10:03:03Z 2023 info:eu-repo/semantics/article Gutiérrez-López, R., Figuerola, J., & Martínez-de la Puente, J. (2022). Methodological procedures explain observed differences in the competence of European populations of Aedes albopictus for the transmission of Zika virus. Acta tropica, 106724. Advance online publication. https://doi.org/10.1016/j.actatropica.2022.106724 https://hdl.handle.net/10481/77610 10.1016/j.actatropica.2022.106724 eng http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Attribution-NonCommercial-NoDerivatives 4.0 Internacional