Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands of Wistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments Segarra, Ana Belén Prieto, Isabel Banegas, Inmaculada Martínez Cañamero, Magdalena Villarejo, Ana B. Domínguez Vías, German Gasparo, Marc de Ramírez Sánchez, Manuel Aminopeptidases Wistar-Kyoto Spontaneously hypertensive rats Pituitary Adrenals Captopril Propranolol L-NAME This research was supported by the Ministry of Science and Innovation through project no. SAF 2008 04685 C02 01. In the present study, we analyzed the activity of several aminopeptidases (angiotensinases) involved in the metabolism of various angiotensin peptides, in pituitary and adrenal glands of untreated Wistar–Kyoto (WKY) and spontaneously hypertensive rats (SHR) or treated with the antihypertensive drugs captopril and propranolol or with the L-Arginine hypertensive analogue L-NG-Nitroarginine Methyl Ester (L-NAME). Intra- and inter-gland correlations between angiotensinase activities were also calculated. Membrane-bound alanyl-, cystinyl-, and glutamylaminopeptidase activities were determined fluorometrically using aminoacyl- -naphthylamide as substrates. Depending on the type of angiotensinase analyzed, the results reflect a complex picture showing substantial differences between glands, strains, and treatments. Alanyl-aminopeptidase responsible for the metabolism of Ang III to Ang IV appears to be the most active angiotensinase in both pituitary and adrenals of WKY and particularly in SHR. Independently of treatment, most positive correlations are observed in the pituitary gland of WKY whereas such positive correlations are predominant in adrenals of SHR. Negative inter-gland correlations were observed in control SHR and L-NAME treated WKY. Positive inter-gland correlations were observed in captopril-treated SHR and propranolol-treated WKY. These results may reflect additional mechanisms for increasing or decreasing systolic blood pressure in WKY or SHR. 2021-09-29T07:00:43Z 2021-09-29T07:00:43Z 2021-07-22 info:eu-repo/semantics/article Segarra, A.B... [et al.]. Interaction between Angiotensinase Activities in Pituitary and Adrenal Glands ofWistar–Kyoto and Spontaneously Hypertensive Rats under Hypotensive or Hypertensive Treatments. Int. J. Mol. Sci. 2021, 22, 7823. [https://doi.org/10.3390/ijms22157823] http://hdl.handle.net/10481/70507 10.3390/ijms22157823 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI