Probiotic and Functional Properties of Limosilactobacillus reuteri INIA P572 Díez Echave, Patricia Garrido-Mesa, José Vezza, Teresa Hidalgo García, Laura Algieri, Francesca Rodríguez Nogales, Alba Rodríguez Cabezas, María Elena Gálvez Peralta, Julio Juan Limosilactobacillus reuteri Probiotics Colonic model DSS-induced colitic model Immunomodulation Reuterin Protective effect Limosilactobacillus reuteri INIA P572 is a strain able to produce the antimicrobial compound reuterin in dairy products, exhibiting a protective effect against some food-borne pathogens. In this study, we investigated some probiotic properties of this strain such as resistance to gastrointestinal passage or to colonic conditions, reuterin production in a colonic environment, and immunomodulatory activity, using different in vitro and in vivo models. The results showed a high resistance of this strain to gastrointestinal conditions, as well as capacity to grow and produce reuterin in a human colonic model. Although the in vitro assays using the RAW 264.7 macrophage cell line did not demonstrate direct immunomodulatory properties, the in vivo assays using a Dextran Sulphate Sodium (DSS)-induced colitic mice model showed clear immunomodulatory and protective effects of this strain. 2021-06-07T11:24:28Z 2021-06-07T11:24:28Z 2021 info:eu-repo/semantics/article Diez-Echave, P.; Martín-Cabrejas, I.; Garrido-Mesa, J.; Langa, S.; Vezza, T.; Landete, J.M.; Hidalgo-García, L.; Algieri, F.; Mayer, M.J.; Narbad, A.; et al. Probiotic and Functional Properties of Limosilactobacillus reuteri INIA P572. Nutrients 2021, 13, 1860. https:// doi.org/10.3390/nu13061860 http://hdl.handle.net/10481/69027 10.3390/nu13061860 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI