Mind the numt: Finding informative mitochondrial markers in a giant grasshopper genome Pereira, Ricardo J. Ruiz-Ruano, Francisco J. Barcoding Mitogenome Numt Orthoptera Pseudogene H2020 Marie Sklodowska-Curie Actions, Grant/Award Number: 658706; Ministerio de Ciencia, Innovacion y Universidades, Grant/Award Number: PID2019-104952GB-I00/AEI/10.13039/501100011033 The barcoding of the mitochondrial COX1 gene has been instrumental in cataloguing the tree of life, and in providing insights in the phylogeographic history of species. Yet, this strategy has encountered difficulties in major clades characterized by large genomes, which contain a high frequency of nuclear pseudogenes originating from the mitochondrial genome (numts). Here, we use the meadow grasshopper (Chorthippus parallelus), which possesses a giant genome of ~13 Gb, to identify mitochondrial genes that are underrepresented as numts, and test their use as informative phylogeographic markers. We recover the same full mitochondrial sequence using both whole genome and transcriptome sequencing, including functional protein‐coding genes and tRNAs. We show that a region of the mitogenome containing the COX1 gene, typically used in DNA barcoding, has disproportionally higher diversity and coverage than the rest of the mitogenome, consistent with multiple insertions of that region into the nuclear genome. By designing new markers in regions of less elevated diversity and coverage, we identify two mitochondrial genes that are less likely to be duplicated as numts. We show that, while these markers show high levels of incomplete lineage sorting between subspecies, as expected for mitochondrial genes, genetic variation reflects their phylogeographic history accurately. These findings allow us to identify useful mitochondrial markers for future studies in C. parallelus, an important biological system for evolutionary biology. More generally, this study exemplifies how non‐PCR‐based methods using next‐generation sequencing can be used to avoid numts in species characterized by large genomes, which have remained challenging to study in taxonomy and evolution. 2021-02-12T12:41:08Z 2021-02-12T12:41:08Z 2020-12-21 info:eu-repo/semantics/article Pereira RJ, Ruiz-Ruano FJ, Thomas CJE, et al. Mind the numt: Finding informative mitochondrial markers in a giant grasshopper genome. J Zool Syst Evol Res. 2020;00:1–11. [https://doi.org/10.1111/jzs.12446] http://hdl.handle.net/10481/66516 10.1111/jzs.12446 eng info:eu-repo/grantAgreement/EC/H2020/Marie Sklodowska-Curie 658706 http://creativecommons.org/licenses/by-nc/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial 3.0 España Wiley