Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines Ripoll, Consuelo Roldán Ortíz, María del Mar Contreras-Montoya, Rafael Díaz Mochón, Juan José Martín Hernández, Miguel Ruedas Rama, María José Orte Gutiérrez, Ángel FLIM microscopy Nanosensing Intracellular sensors Tumoral metabolism Cancer metabolism We thank the company Nanogetic S.L. (Granada, Spain) for support with the synthesis of the SS peptides and the Centro de Instrumentacion Cientifica (CIC) of the Universidad de Granada for use of the TEM facilities. The main role of mitochondria, as pivotal organelles for cellular metabolism, is the production of energy (ATP) through an oxidative phosphorylation system. During this process, the electron transport chain creates a proton gradient that drives the synthesis of ATP. One of the main features of tumoral cells is their altered metabolism, providing alternative routes to enhance proliferation and survival. Hence, it is of utmost importance to understand the relationship between mitochondrial pH, tumoral metabolism, and cancer. In this manuscript, we develop a highly specific nanosensor to accurately measure the intramitochondrial pH using fluorescence lifetime imaging microscopy (FLIM). Importantly, we have applied this nanosensor to establish differences that may be hallmarks of different metabolic pathways in breast cancer cell models, leading to the characterization of different metabophenotypes. 2020-07-28T11:52:34Z 2020-07-28T11:52:34Z 2020-05 info:eu-repo/semantics/article Ripoll, C., Roldan, M., Contreras-Montoya, R., Diaz-Mochon, J. J., Martin, M., Ruedas-Rama, M. J., & Orte, A. (2020). Mitochondrial pH Nanosensors for Metabolic Profiling of Breast Cancer Cell Lines. International Journal of Molecular Sciences, 21(10), 3731. [DOI: 10.3390/ijms21103731] http://hdl.handle.net/10481/63171 10.3390/ijms21103731 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI