Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots Murru, Fabio Romero Maldonado, Francisco Javier Sánchez Mudarra, Roberto García Ruiz, Francisco Javier Morales Santos, Diego Pedro Capitán Vallvey, Luis Fermín Salinas Castillo, Alfonso Romero Maldonado, Francisco Javier Carbon dots Hemoglobin determination Luminescence Room-temperature phosphorescence Portable instrument A portable reconfigurable platform for hemoglobin determination based on inner filter quenching of room-temperature phosphorescent carbon dots (CDs) in the presence of H2O2 is described. The electronic setup consists of a light-emitting diode (LED) as the carbon dot optical exciter and a photodiode as a light-to-current converter integrated in the same instrument. The reconfigurable feature provides adaptability to use the platform as an analytical probe for CDs coming from different batches with some variations in luminescence characteristics. The variables of the reaction were optimized, such as pH, concentration of reagents, and response time; as well as the variables of the portable device, such as LED voltage, photodiode sensitivity, and adjustment of the measuring range by a reconfigurable electronic system. The portable device allowed the determination of hemoglobin with good sensitivity, with a detection limit of 6.2 nM and range up to 125 nM. 2020-07-14T11:43:07Z 2020-07-14T11:43:07Z 2020-04 info:eu-repo/semantics/article Murru, F., Romero, F. J., Sánchez-Mudarra, R., García Ruiz, F. J., Morales, D. P., Capitán-Vallvey, L. F., & Salinas-Castillo, A. (2020). Portable Instrument for Hemoglobin Determination Using Room-Temperature Phosphorescent Carbon Dots. Nanomaterials, 10(5), 825. [doi:10.3390/nano10050825] http://hdl.handle.net/10481/62971 10.3390/nano10050825 eng http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Atribución 3.0 España MDPI