Silver improves collagen structure and stability at demineralized dentin: a dynamic-mechanical and Raman analysis. Osorio Ruiz, María Estrella Toledano Osorio, Manuel Osorio Ruiz, Raquel Sánchez Aguilera, Fátima Medina Castillo, Antonio Luis Toledano Pérez, Manuel Toledano Osorio, Manuel Collagen Erosion Demineralisation Dentine Microscopy Raman Objective: This study aimed to evaluate the effect of silver loaded nanoparticles (NPs) application on dentin remineralization. Methods: Polymethylmetacrylate-based NPs and silver loaded NPs (Ag-NPs) were applied on demineralized dentin surfaces. Dentin was characterized morphologically by scanning electron microscopy, mechanically probed by a nanoindenter to test nanohardness and Young modulus, and chemically analyzed by Raman spectroscopy after 24 h and 7 d of storage. Untreated surfaces were used as control. Data were submitted to ANOVA and Student-Newman-Keuls multiple comparisons tests (P<0.05). Results: After Raman analysis, dentin treated with Ag-NPs obtained the lowest mineralization and intensity of stoichiometric hydroxyapatite when compared with specimens treated with undoped-NPs. The lowest relative mineral concentration, expressed as the ratio phosphate or carbonate/phenyl group, and crystallinity was attained by dentin treated with with Ag-NPs, after 7 d. Ag-NPs application generated the highest values of collagen crosslinking (intensity at 1032 cm-1 band). The molecular conformation of the collagen’s polypeptide chains, amide-I and CH2 also attained the highest peaks in dentin treated with Ag-NPs. Staggered and demineralized collagen fibrils were observed covering the dentin surfaces treated with Ag-NPs, at both 24 h and 7 d. Samples treated with Ag-NPs attained the lowest values of nanohardness and Young’s modulus at 7 d of storage. Conclusions: Peritubular and intertubular dentin were remineralized when using undoped-NPs. After 7 d, collagen treated with NPs was remineralized but dentin treated with Ag-NPs attained an improved collagen matrix structure and stability but the lowest mineralization and crystallinity. 2018-11-08T10:14:18Z 2018-11-08T10:14:18Z 2018-10 info:eu-repo/semantics/article Osorio R, Osorio E, Aguilera FS, Medina-Castillo AL, Toledano M, Toledano-Osorio M. Silver improves collagen structure and stability at demineralized dentin: A dynamic-mechanical and Raman analysis. J Dent. 2018 Oct 5. pii: S0300-5712(18)30537-2. [http://hdl.handle.net/10481/53637] http://hdl.handle.net/10481/53637 10.1016/j.jdent.2018.10.003 eng http://creativecommons.org/licenses/by-nc-nd/3.0/es/ info:eu-repo/semantics/openAccess Atribución-NoComercial-SinDerivadas 3.0 España