Additive Effects of Threat-of-Shock and Picture Valence on Startle Reflex Modulation Bublatzky, Florian Guerra Muñoz, Pedro María Pastor, María del Carmen Schupp, Harald T. Vila Castellar, Jaime Anxiety Anxiety disorders Data processing Fear Human learning Reflexes Safety studies Sensory cues The present study examined the effects of sustained anticipatory anxiety on the affective modulation of the eyeblink startle reflex. Towards this end, pleasant, neutral and unpleasant pictures were presented as a continuous stream during alternating threat-of-shock and safety periods, which were cued by colored picture frames. Orbicularis-EMG to auditory startle probes and electrodermal activity were recorded. Previous findings regarding affective picture valence and threat-of-shock modulation were replicated. Of main interest, anticipating aversive events and viewing affective pictures additively modulated defensive activation. Specifically, despite overall potentiated startle blink magnitude in threat-of-shock conditions, the startle reflex remained sensitive to hedonic picture valence. Finally, skin conductance level revealed sustained sympathetic activation throughout the entire experiment during threat- compared to safety-periods. Overall, defensive activation by physical threat appears to operate independently from reflex modulation by picture media. The present data confirms the importance of simultaneously manipulating phasic-fear and sustained-anxiety in studying both normal and abnormal anxiety. 2014-03-19T10:58:38Z 2014-03-19T10:58:38Z 2013 info:eu-repo/semantics/article Bublatzky, F.; et al. Additive Effects of Threat-of-Shock and Picture Valence on Startle Reflex Modulation. Plos One, 8(1): e54003 (2013). [] 1932-6203 doi:10.1371/journal.pone.0054003 http://hdl.handle.net/10481/30967 eng http://creativecommons.org/licenses/by-nc-nd/3.0/ info:eu-repo/semantics/openAccess Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License Public Library of Science (PLOS)