Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs

González-Pozas, Federico; Montes, Rosa; López Onieva, Lourdes; Romero, Tamara; Domingo Reinés, Joan; Real Luna, Pedro José; Ramos Mejía, Verónica; Ayllon, Verónica

doi:10.1016/j.scr.2016.04.005

Gonzalez-Pozas et al. Stem Cell Research 2016.pdf (1.025Mo)

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URI: https://hdl.handle.net/10481/98874

DOI: 10.1016/j.scr.2016.04.005

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Date

2016-05

Referencia bibliográfica

González-Pozas F, Montes R, López-Onieva L, Romero T, Domingo-Reinés J, Real PJ, Ramos-Mejía V, Ayllón V. Generation of human pluripotent stem cell lines with suppressed expression of the Notch ligand DLL4 using short hairpin RNAs. Stem Cell Res. 2016 May;16(3):735-9. doi: 10.1016/j.scr.2016.04.005. Epub 2016 Apr 16. PMID: 27346199.

Résumé

Studies with different animal models have shown that the Notch ligand DLL4 has a key role in the development of the embryonic vasculature. Here we describe the generation and characterization of a human embryonic stem cell line and an induced pluripotent stem cell line that constitutively express short hairpin RNAs targeting DLL4 mRNA. These cells present reduced DLL4 expression at both protein and mRNA level, as well as a reduced induction of DLL4 target genes. They represent an ideal tool to study the role of DLL4 in human embryonic vascular and hematopoietic development.

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