miR-122 direct detection in human serum by time-gated fluorescence imaging

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URI: https://hdl.handle.net/10481/97955
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Autor
García Fernández, Emilio; González García, María del Carmen; Pernagallo, Salvatore; Ruedas-Rama, Maria Jose; Fara, Mario Antonio; López Delgado, Francisco Javier; W. Dear, James; Ilyine, Hugh; Ress, Cristina; Díaz Mochón, Juan José; Orte Gutiérrez, Ángel
Editorial
Royal Society of Chemistry
Fecha
2019-11-27
Referencia bibliográfica
García Fernández, E. et. al. Chem. Commun., 2019, 55, 14958. [https://doi.org/10.1039/C9CC08069D]
Resumen
A simple method for direct detection of microRNAs (miRs) in human serum without the use of polymerase amplification is presented, achieving low miR-122 concentrations and importantly, discerning effectively single-base sequence mutations. The method is based on the capture of target miRs with synthetic peptide nucleic acid oligomers, dynamic chemical labelling, separation with quaternary amine microplatforms and detection using time-gated fluorescence imaging.
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Atribución 4.0 Internacional
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