Doxycycline-doped membranes induced osteogenic gene expression on osteoblastic cells
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Toledano Osorio, Manuel; Manzano-Moreno, Francisco Javier; Toledano Pérez, Manuel; Osorio Ruiz, Raquel; Medina Castillo, Antonio Luis; Costela Ruiz, Víctor Javier; Ruiz Rodríguez, ConcepciónEditorial
Elsevier
Materia
RT-qPCR Doxycycline Gene expression GBR Membrane
Date
2021-06Referencia bibliográfica
M. Toledano-Osorio et al. Doxycycline-doped membranes induced osteogenic gene expression on osteoblastic cells. Journal of Dentistry 109 (2021) 103676. https://doi.org/10.1016/j.jdent.2021.103676
Sponsorship
Ministry of Economy and Competitiveness-European Regional Development Fund[Project MAT2017-85999-P MINECO/AEI/FEDER/UE]; University of Granada/Regional Government of Andalusia (A-BIO-157-UGR-18/FEDER)Abstract
Objectives: To analyze how novel developed silicon dioxide composite membranes, functionalized with zinc or
doxycycline, can modulate the expression of genes related to the osteogenic functional capacity of osteoblastic
cells.
Methods: The composite nanofibers membranes were manufactured by using a novel polymeric blend and 20 nm
silicon dioxide nanoparticles (SiO2-NPs). To manufacture the membranes, 20 nm SiO2-NPs were added to the
polymer solution and the resulting suspension was processed by electrospinning. In a second step, the membranes were functionalized with zinc or doxycycline. Then, they were subjected to MG63 osteoblast-like cells
culturing for 48 h. After this time, real-time quantitative polymerase chain reaction (RT-qPCR) was carried out to
study the expression of Runx-2, OSX, ALP, OSC, OPG, RANKL, Col-I, BMP-2, BMP-7, TGF-β1, VEGF, TGF-βR1,
TGF- βR2, and TGF-βR3. Mean comparisons were conducted by One-way ANOVA and Tukey tests (p < 0.05).
Results: In general, the blending of SiO2-NPs in the tested non-resorbable polymeric scaffold improves the
expression of osteogenic genes over the control membranes. Doxycycline doping of experimental scaffolds
attained the best results, encountering up-regulation of BMP-2, ALP, OPG, TGFβ-1 and TGFβ-R1. Membranes
with zinc induced a significant increase in the expression of Col-I, ALP and TGF β1. Both, zinc and doxycycline
functionalized membranes enormously down-regulated the expression of RANKL.
Conclusions: Zinc and doxycycline doped membranes are bioactive inducing overexpression of several osteogenic
gene markers.
Clinical significance: Doxycycline doped membranes may be a potential candidate for use in GBR procedures in
several challenging pathologies, including periodontal diseases.