Comparison of an Enzyme Linked-Immunosorbent Assay and a Chemiluminescent Immunoassay with an Immunofluorescence Assay for Detection of Phase II IgM and IgG Antibodies to Coxiella burnetii
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Gutiérrez Bautista, Juan Francisco; Tarriño, María; González, Adrián; Olivares Durán, María José; Cobo, Fernando; Reguera, Juan Antonio; Rodríguez-Granger, Javier; SAmpedro, AntonioEditorial
MDPI
Materia
Coxiella burnetii Q fever Diagnosis
Date
2024-03-11Referencia bibliográfica
Gutiérrez-Bautista, J.F.; Tarriño, M.; González, A.; Olivares Durán, M.J.; Cobo, F.; Reguera, J.A.; Rodríguez-Granger, J.; Sampedro, A. Comparison of an Enzyme Linked-Immunosorbent Assay and a Chemiluminescent Immunoassay with an Immunofluorescence Assay for Detection of Phase II IgM and IgG Antibodies to Coxiella burnetii. Microorganisms 2024, 12, 552. https://doi.org/10.3390/microorganisms12030552
Abstract
In this study, we have compared the detection of IgM and IgG against C. burnetii phase II of
an enzyme-linked immunosorbent assay (ELISA) (Euroimmun) and a chemiluminescent immunoassay
(CLIA) (VIRCLIA, Vircell). In addition, an indirect immunofluorescence assay (IFA) was used as
a reference test. One hundred forty-eight sera were used for IgG evaluation, and eighty-eight for IgM.
The sensitivity of ELISA and CLIA in detecting phase II IgM was excellent. On the other hand, the
CLIA IgM showed better specificity than the ELISA IgM. As for phase II IgG, the specificity of ELISA
and CLIA was similar, while the ELISA technique showed a higher sensitivity. In conclusion, the
best system to detect phase II IgM antibodies against C. burnetii is the CLIA from Vircell, which is
characterized by high sensitivity and specificity. For the detection of phase II IgG, the Euroimmun
ELISA and Vircell CLIA assays are suitable for the determination of this marker in the laboratory,
although the IgG ELISA has greater sensitivity.