Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype
Metadata
Show full item recordAuthor
Goñi, Pilar; Almagro-Nievas, Diego; Cieloszyk, Joanna; Lóbez, Silvia; Navarro Marí, José María; Gutiérrez Fernández, José; Lóbez, SilviaEditorial
Elsevier
Materia
Cryptosporidium Giardia Outbreak IaA11R2 IbA10G2R2 Assemblage
Date
2015Referencia bibliográfica
Published version: Goñi P, Almagro-Nievas D, Cieloszyk J, Lóbez S, Navarro-Marí JM, Gutiérrez-Fernández J. Cryptosporidiosis outbreak in a child day-care center caused by an unusual Cryptosporidium hominis subtype. Enferm Infecc Microbiol Clin. 2015 Dec;33(10):651-5. DOI: 10.1016/j.eimc.2015.02.003
Sponsorship
University of Zaragoza: DGA-FSE Research Team B82, UZ2013-FIS-02Abstract
Introduction: This work describes the genetic characterization of Cryptosporidium and Giardia involved in an outbreak in a nursery school in Granada, Spain, that affected seven children under the age of 4.
Methods: Nucleic acids were extracted from the seven stool samples positive to Cryptosporidium or Giardia by microscopy and/or immunochromatography. The species and subtypes of Cryptosporidium were identified by PCR-RFLP and PCR of the SSUrRNA and gp60 genes, respectively. The assemblages of Giardia duodenalis isolates were characterized by PCR of the tpi gene. PCR products were sequenced and analyzed.
Results: All of the isolates were positive for Cryptosporidium hominis. Five of them belonged to subtype IaA11R2, one to subtype IbA10G2R2, and the other could not be identified. Three of these samples were positive for G. duodenalis by PCR, two belonging to the assemblage A, and the other one to assemblage B.
Discussion: This is the first report of Cryptosporidium hominis subtype IaA11R2 as a cause of an outbreak in Europe where subtype IbA10G2R2 is the most frequently identified. In the case of Giardia, an outbreak could not be confirmed because of the low number of positive samples and the low genetic variability of the amplified fragments for assemblage A of tpi gene.
Conclusions: A new subtype, of Cryptosporidium hominis named IaA11R2, has been described as a cause of an outbreak in a nursery school in Granada, Spain. However an outbreak of giardiasis could not be confirmed.