Metal-dependent Glycosylation in Recombinant Metallothioneins

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URI: https://hdl.handle.net/10481/90378
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Author
García Risco, Mario; González Garnica, Ana Isabel; Calatayud, Sara; López Jaramillo, Francisco Javier; Pedrini Martha, Veronika; Albalat, Ricard; Dallinger, Reinhard; Domínguez Vera, José Manuel; Palacios, Óscar; Capdevila, Mercè
Editorial
Royal Society of Chemistry
Date
2022-11-15
Referencia bibliográfica
Published version: García Risco, Mario et al. Metal-dependent Glycosylation in Recombinant Metallothioneins. Chem. Commun., 2022,58, 13755-13758. https://doi.org/10.1039/D2CC05589A
Sponsorship
Spanish Ministerio de Ciencia e Innovación; FEDER: BIO2015-67358-C2-2-P, PID2021-123258NB-I00, PID2019-111461GB-I00; Austrian Science Fund I 3032-B21; Generalitat de Catalunya 2017SGR-864, 2017SGR-1665; Junta de Andalucía PAIDI 2020 program (DOC_00791); Universitat Autònoma de Barcelona (ICP-AES, ESI-MS); University of Innsbruck
Abstract
We show for the first time glycosylation of recombinant metallothioneins (MTs) produced in E. coli. Interestingly, our results show that the glycosylation level of the recombinant MTs is inversely proportional to the degree of protein structuration, and reflects their different metal preferences.
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