The endoplasmic reticulum Ca2+-ATPase SERCA2b is upregulated in activated microglia and its inhibition causes opposite effects on migration and phagocytosis
Metadatos
Afficher la notice complèteAuteur
Morales-Ropero, Juan M; Arroyo-Urea, Sandra; Neubrand, Veronika Elisabeth; Martín Oliva, Francisco David; Marín Teva, José Luis; Cuadros Ojeda, Miguel Ángel; Vangheluwe, Peter; Navascues Martínez, Julio; Mata, Ana M; Sepúlveda Justo, María Del RosarioEditorial
Wiley
Materia
Alzheimer's disease Brain Calcium pump
Date
2021Referencia bibliográfica
Glia 69: 842–857
Patrocinador
Grant mP_BS_35-2014 from CEI BioTic Granada; PP2016-PJI05 from University of Granada; A1-CTS-324-UGR18 from FEDER-Junta de Andalucía, Spain; PP2016-PIP08 from University of Granada; BFU2017-85723-P from Spanish Ministry of Economy and Competitiveness co-financed with FEDER; G044212N from Flanders Research FoundationRésumé
Activation of microglia is an early immune response to damage in the brain. Although a key role for Ca2+ as trigger of microglial activation has been considered, little is known about the molecular scenario for regulating Ca2+ homeostasis in these cells. Taking into account the importance of the endoplasmic reticulum as a cellular Ca2+ store, the sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA2b) is an interesting target to modulate intracellular Ca2+ dynamics. We found upregulation of SERCA2b in activated microglia of human brain with Alzheimer´s disease and we further studied the participation of SERCA2b in microglial functions by using the BV2 murine microglial cell line and primary microglia isolated from mouse brain. To trigger microglia activation, we used the bacterial lipopolysaccharide (LPS), which is known to induce an increase of cytosolic Ca2+. Our results showed an upregulated expression of SERCA2b in LPS-induced activated microglia likely associated to an attempt to restore the increased cytosolic Ca2+ concentration. We analyzed SERCA2b contribution in microglial migration by using the specific SERCA inhibitor thapsigargin in scratch assays. Microglial migration was strongly stimulated with thapsigargin, even more than with LPS-induction, but delayed in time. However, phagocytic capacity of microglia was blocked in the presence of the SERCA inhibitor, indicating the importance of a tight control of cytosolic Ca2+ in these processes. All together, these results provide for the first time compelling evidence for SERCA2b as a major player regulating microglial functions, affecting migration and phagocytosis in an opposite manner.