Stenotrophomonas bentonitica sp. nov., isolated from bentonite formations
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Ruiz Fresneda, Miguel ÁngelDate
2017Abstract
A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7T
, was isolated during a study targeting the culturedependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence
analysis showed that BII-R7T
represented a member of the genus Stenotrophomonas (class Gammaproteobacteria), and was
related most closely to Stenotrophomonas rhizophila e-p10T
(99.2 % sequence similarity), followed by Stenotrophomonas
pavanii ICB 89T
(98.5 %), Stenotrophomonas maltophilia IAM 12423T
, Stenotrophomonas chelatiphaga LPM-5T
and
Stenotrophomonas tumulicola T5916-2-1bT
(all 98.3 %). Pairwise sequence similarities to all other type strains of species of
the genus Stenotrophomonas were below 98 %. Genome-based calculations (orthologous average nucleotide identity, original
average nucleotide identity, genome-to-genome distance and DNA G+C percentage) indicated clearly that the isolate
represents a novel species within this genus. Different phenotypic analyses, such as the detection of a quinone system
composed of the major compound ubiquinone Q-8 and a fatty acid profile with iso-C15 : 0 and anteiso-C15 : 0 as major
components, supported this finding at the same time as contributing to a comprehensive characterization of BII-R7T
. Based
on this polyphasic approach comprising phenotypic and genotypic/molecular characterization, BII-R7T
can be differentiated
clearly from its phylogenetic neighbours, establishing a novel species for which the name Stenotrophomonas bentonitica sp.
nov. is proposed with BII-R7T
as the type strain (=LMG 29893T
=CECT 9180T
=DSM 103927T
).