Exchangeable Self-Assembled Lanthanide Antennas for PLIM Microscopy
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Ruiz Arias, Álvaro; Fueyo-Gonzalez, Francisco; Izquierdo-Garcia, Carolina; Navarro, Amparo; Gutierrez-Rodriguez, Marta; Herranz, Rosario; Burgio, Chiara; Reinoso, Antonio; Cuerva Carvajal, Juan Manuel; Orte Gutiérrez, Ángel; González Vera, Juan AntonioEditorial
Wiley
Date
2023Referencia bibliográfica
Angew. Chem. Int. Ed. 2023, e202314595 doi.org/10.1002/anie.202314595
Sponsorship
Grant PID2020-114256RB-I00 funded by AEI/10.13039/501100011033; Grant PID2019- 104366RB-C22 funded by AEI/10.13039/501100011033/ FEDER “Una manera de hacer Europa”; Grants P21_00212, A-FQM-386-UGR20 and 2021/00627/001-FEDER_UJA_ 2020 funded by FEDER/Junta de Andalucía-Consejería de Transformación Económica, Industria, Conocimiento y Universidades; CSIC grant 202180E073; Acción 1 from Universidad de Jaén; Funding for open access charge: Universidad de Granada/CBUA; Spanish Ministerio de Educación y Formación Profesional for the FPU Ph.D. scholarshipAbstract
Lanthanides have unique photoluminescence (PL) emission properties, including very long PL life- times. This makes them ideal for biological imaging applications, especially using PL lifetime imaging micro- scopy (PLIM). PLIM is an inherently multidimensional technique with exceptional advantages for quantitative biological imaging. Unfortunately, due to the required prolonged acquisitions times, photobleaching of lantha- nide PL emission currently constitutes one of the main drawbacks of PLIM. In this study, we report a small aqueous-soluble, lanthanide antenna, 8-methoxy-2-oxo- 1,2,4,5-tetrahydrocyclopenta[de]quinoline-3-phosphonic acid, PAnt, specifically designed to dynamically interact with lanthanide ions, serving as exchangeable dye aimed at mitigating photobleaching in PLIM microscopy in cellulo. Thus, self-assembled lanthanide complexes that may be photobleached during image acquisition are continuously replenished by intact lanthanide antennas from a large reservoir. Remarkably, our self-assembled lanthanide complex clearly demonstrated a significant reduction of PL photobleaching when compared to well- established lanthanide cryptates, used for bioimaging. This concept of exchangeable lanthanide antennas opens new possibilities for quantitative PLIM bioimaging.