Inflammasomes NLRP3 and AIM2 in peri-implantitis: A cross-sectional study
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Galindo Moreno, Pablo Antonio; Montalvo Acosta, Saray; Martín Morales, Natividad; Carrillo Gálvez, Ana Belén; González-Rey, Elena; O'Valle Ravassa, Francisco Javier; Padial Molina, MiguelEditorial
Wiley
Materia
AIM2 Inflammasome Inflammation NLRP3 Peri-implant disease Peri-implantitis
Date
2023-08-29Referencia bibliográfica
Galindo‐Moreno, P., Montalvo‐Acosta, S., Martín‐Morales, N., Carrillo‐Gálvez, A. B., González‐Rey, E., O'Valle, F., & Padial‐Molina, M. (2023). Inflammasomes NLRP3 and AIM2 in peri‐implantitis: A cross‐sectional study. Clinical Oral Implants Research.[DOI: 10.1111/clr.14174]
Sponsorship
Junta de Andalucía, Grant/Award Number: CTS-138; CTS-1028; Universidad de Granada, Grant/Award Number: B-CTS- 504- UGR18; Universidad de Granada/CBUAAbstract
Background: Inflammasome components NLRP3 and AIM2 contribute to inflammation
development by the activation of caspase-1
and IL-1β.
They have not been yet
evaluated in samples from patients with active peri-implantitis.
Thus, the aim of the
present study is to analyze the expression of inflammasomes NLRP3 and AIM2 and
subsequent caspase 1 and IL-1β
assessing the microenvironment of leukocyte subsets
in samples from patients with active peri-implantitis.
Methods: Biopsies were collected from 33 implants in 21 patients being treated for
peri-implantitis.
Biopsies from gingival tissues from 15 patients with healthy periodontium
were also collected for control. These tissues were evaluated through conventional
histological stainings. Then, immunohistochemical detection was performed to
analyze NLRP3, AIM2, caspase-1,
and IL-1β
and markers of different leukocyte subsets.
PCR for inflammasomes and related genes was also done.
Results: This manuscript reveals a high immunohistochemical and mRNA expression
of NLRP3 and AIM2 inflammasomes, caspase-1,
and IL-1β
in biopsies collected from
human peri-implantitis.
The expression of the tested markers was significantly correlated
with the increase in inflammatory infiltrate, probing depth, presence of biofilm,
and bleeding on probing. In these peri-implantitis
lesions, the area of biopsy tissue occupied
by inflammatory infiltrate was intense while the area occupied by collagen was
significantly lower. In comparison with periodontal healthy tissues, the inflammatory
infiltrate was statistically significantly higher in the peri-implantitis
biopsies and was
mainly composed of plasma cells, followed by T and B lymphocytes.
Conclusion: In human peri-implantitis,
chronic inflammation can be explained in part
by the action of IL-1β/
caspase 1 induced through NLRP3 and AIM2 inflammasome
activation.