A comparison between petrous bone and tooth, femur and tibia DNA analysis from degraded skeletal remains
Metadatos
Afficher la notice complèteAuteur
Haarkötter Cardoso, Christian; Gálvez Escolano, Xiomara; Sáiz Guinaldo, María; Medina Lozano, María Isabel; Lorente Acosta, José Antonio; Álvarez Merino, Juan CarlosEditorial
Wiley
Materia
Degraded DNA Forensic genetics Human remains Petrous bone Skeletal remains
Date
2023-07-19Referencia bibliográfica
Haarkötter, C., Vinueza‐Espinosa, D. C., Gálvez, X., Saiz, M., Medina‐Lozano, M. I., Lorente, J. A., & Álvarez, J. C. (2023). A comparison between petrous bone and tooth, femur and tibia DNA analysis from degraded skeletal remains. Electrophoresis.[DOI: 10.1002/elps.202300097]
Résumé
Skeletal remains are the only biological material that remains after long periods;
however, environmental conditions such as temperature, humidity, andpHaffect
DNA preservation, turning skeletal remains into a challenging sample for DNA
laboratories. Sample selection is a key factor, and femur and tooth have been
traditionally recommended as the best substrate of genetic material. Recently,
petrous bone (cochlear area) has been suggested as a better option due to its
DNA yield. This research aims to evaluate the efficiency of petrous bone compared
to other cranium samples (tooth) and postcranial long bones (femur and
tibia). A total amount of 88 samples were selected from 38 different individuals.
The samples were extracted by using an organic extraction protocol, DNA quantification
by Quantifiler Trio kit and amplified with GlobalFiler kit. Results show
that petrous bone outperforms other bone remains in quantification data, yielding
15–30 times more DNA than the others. DNA profile data presented likeness
between petrous bone and tooth regarding detected alleles; however, the amount
of DNA extracted in petrous bones allowed us to obtain more informative DNA
profiles with superior quality. In conclusion, petrous bone or teeth sampling is
recommended if DNA typing is going to be performed with environmentally
degraded skeletal remains.