Development of secretome‑based strategies to improve cell culture protocols in tissue engineering
Metadatos
Mostrar el registro completo del ítemAutor
Cases Perera, Oriol; Blanco Elices, Cristina; Chato Astrain, Jesús; Miranda‑Fernández, C.; Fernández Campos, F.; Crespo Ferrer, Pascual Vicente; Sánchez-Montesinos García, Indalecio; Alaminos Mingorance, Miguel; Martín Piedra, Miguel Ángel; Garzón Bello, Ingrid JohannaEditorial
Nature Portfolio
Fecha
2022-06-15Referencia bibliográfica
O. Cases‑Perera et al. Development of secretome‑based strategies to improve cell culture protocols in tissue engineering. Scientific Reports (2022) 12:10003. [https://doi.org/10.1038/s41598-022-14115-y]
Patrocinador
Spanish Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I + D + I) of the Spanish Ministry of Science and Innovation (Instituto de Salud Carlos III) FIS PI18/0331, FIS PI21/0980, FIS PI18/0332, FIS PI20/0317, ICI19/00024, ICI21/00010; Junta de Andalucia PE-0395-2019, PI-0442-2019; Proyectos de I + D + i en el marco del Programa Operativo FEDER Andalucia , University of Granada and Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades B-CTS-450-UGR20; European CommissionResumen
Advances in skin tissue engineering have promoted the development of artificial skin substitutes
to treat large burns and other major skin loss conditions. However, one of the main drawbacks to
bioengineered skin is the need to obtain a large amount of viable epithelial cells in short periods of
time, making the skin biofabrication process challenging and slow. Enhancing skin epithelial cell
cultures by using mesenchymal stem cells secretome can favor the scalability of manufacturing
processes for bioengineered skin. The effects of three different types of secretome derived
from human mesenchymal stem cells, e.g. hADSC‑s (adipose cells), hDPSC‑s (dental pulp) and
hWJSC‑s (umbilical cord), were evaluated on cultured skin epithelial cells during 24, 48, 72 and 120 h
to determine the potential of this product to enhance cell proliferation and improve biofabrication
strategies for tissue engineering. Then, secretomes were applied in vivo in preliminary analyses carried
out on Wistar rats. Results showed that the use of secretomes derived from mesenchymal stem cells
enhanced currently available cell culture protocols. Secretome was associated with increased viability,
proliferation and migration of human skin epithelial cells, with hDPSC‑s and hWJSC‑s yielding greater
inductive effects than hADSC‑s. Animals treated with hWJSC‑s and especially, hDPSC‑s tended to
show enhanced wound healing in vivo with no detectable side effects. Mesenchymal stem cells derived
secretomes could be considered as a promising approach to cell‑free therapy able to improve skin
wound healing and regeneration.