Maternal urinary concentrations of bisphenol A during pregnancy are associated with global DNA methylation in cord blood of newborns in the “NELA” birth cohort
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Navarro Lafuente, Fuensanta; Fernández Cabrera, Mariana Fátima; Suárez González, Beatriz; NELA Study GroupEditorial
Elsevier
Materia
Bisphenol A DNA methylation LINE-1 Perinatal exposures
Date
2022-06-07Referencia bibliográfica
Fuensanta Navarro-Lafuente... [et al.]. Maternal urinary concentrations of bisphenol A during pregnancy are associated with global DNA methylation in cord blood of newborns in the “NELA” birth cohort, Science of The Total Environment, Volume 838, Part 4, 2022, 156540, ISSN 0048-9697, [https://doi.org/10.1016/j.scitotenv.2022.156540]
Sponsorship
Instituto de Salud Carlos III (ISCIII), Spanish Ministry of Science, Innovation and Universities, Fondos FEDER MS14/00046 CP14/00046 PIE15/00051 PI16/00422 FI17/00086 PI19/00863; Fundacion Seneca, Agencia de Ciencia y Tecnologia Region de Murcia 20877/PI/18; Ministry of Science and Innovation, Spain (MICINN); Spanish Government FPU18/01990; ISCIII, Spanish Ministry of Science, Innovation and Universities, and Fondos FEDER MS14/00046 CPII19/00019Abstract
Endocrine disrupting chemicals (EDCs) set a public health risk through disruption of normal physiological processes.
The toxicoepigeneticmechanisms of developmental exposure to common EDCs, such as bisphenol A (BPA), are poorly
known. The present study aimed to evaluate associations between perinatal maternal urinary concentrations of BPA,
bisphenol S (BPS) and bisphenol F (BPF) and LINE-1 (long interspersed nuclear elements) and Alu (short interspersed
nuclear elements, SINEs) DNA methylation levels in newborns, as surrogate markers of global DNA methylation. Data
come from 318 mother-child pairs of the `Nutrition in Early Life and Asthma´ (NELA) birth cohort. Urinary bisphenol
concentrationwas measured by dispersive liquid–liquid microextraction and ultrahigh performance liquid chromatography
with tandem mass spectrometry detection. DNA methylation was quantitatively assessed by bisulphite pyrosequencing
on 3 LINEs and 5 SINEs. Unadjusted linear regression analyses showed that higher concentration ofmaternal
urinary BPA in 24th week's pregnancy was associated with an increase in LINE-1 methylation in all newborns (p =
0.01) and, particularly, in male newborns (p = 0.03). These associations remained in full adjusted models [beta =
0.09 (95 % CI = 0.03; 0.14) for all newborns; and beta= 0.10 (95 % CI = 0.03; 0.17) for males], including a non-linear association for female newborns as well (p-trend=0.003). No associations were found between maternal concentrations
of bisphenol and Alu sequences. Our results suggest that exposure to environmental levels of BPA may be
associated with a modest increase in LINE-1 methylation -as a relevant marker of epigenomic stability- during human
fetal development. However, any effects on global DNA methylation are likely to be small, and of uncertain biological
significance.