A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime imaging
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Fernández, Antonio; González García, María del Carmen; Espinar Barranco, Laura; González Vera, Juan Antonio; Orte Gutiérrez, ÁngelEditorial
Royal Society of Chemistry
Date
2022-08-25Referencia bibliográfica
RSC Chem. Biol., 2022, Advance Article. DOI: [10.1039/d2cb00168c]
Sponsorship
Fundacion Seneca 21124/SF/19 MCIN/AEI PID2020-114256RB-I00; Spanish Government; European Commission PID2019-107991RB-I00 European Research Council (ERC) European Commission 771443; Royal Society of London; European Commission RG160289Abstract
We report a platform combining multicomponent reaction synthesis and automated cell-based
screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores,
we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED superresolution
and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging
trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic
between intracellular compartments and the plasma membrane.