Structure of fungal communities in sequencing batch reactors operated at different salinities for the selection of triacylglyceride-producers from a fish-canning lipid-rich waste stream
Metadatos
Afficher la notice complèteAuteur
Correa Galeote, David; Juárez Jiménez, María José Belén; González López, Jesús Juan; Rodelas González, María BelénEditorial
Elsevier
Materia
TAG-accumulation Fish-canning WWTP QPCR Fungal diversity Network correlation analysis Candida Sequencing batch reactors
Date
2022-08-02Referencia bibliográfica
David Correa-Galeote... [et al.]. Structure of fungal communities in sequencing batch reactors operated at different salinities for the selection of triacylglyceride-producers from a fish-canning lipid-rich waste stream, New Biotechnology, Volume 71, 2022, Pages 47-55, ISSN 1871-6784, [https://doi.org/10.1016/j.nbt.2022.08.001]
Patrocinador
Spanish Government CTQ2017 - 83225-C2 - 1- R CTQ2017 - 83225-C2 - 2-R PID-2020 - 112550RB-C22Résumé
Oleaginous fungi natively accumulate large amounts of triacylglycerides (TAG), widely used as precursors for
sustainable biodiesel production. However, little attention has been paid to the diversity and roles of fungal
mixed microbial cultures (MMCs) in sequencing batch reactors (SBR). In this study, a lipid-rich stream produced
in the fish-canning industry was used as a substrate in two laboratory-scale SBRs operated under the feast/famine
(F/F) regime to enrich microorganisms with high TAG-storage ability, under two different concentrations of NaCl
(SBR-N: 0.5 g/L; SBR-S: 10 g/L). The size of the fungal community in the enriched activated sludge (EAS) was
analyzed using 18S rRNA-based qPCR, and the fungal community structure was determined by Illumina
sequencing. The different selective pressures (feeding strategy and control of pH) implemented in the enrichment
SBRs throughout operation increased the abundance of total fungi. In general, there was an enrichment of genera
previously identified as TAG-accumulating fungi (Apiotrichum, Candida, Cutaneotrichosporon, Geotrichum,
Haglerozyma, Metarhizium, Mortierella, Saccharomycopsis, and Yarrowia) in both SBRs. However, the observed
increase of their relative abundances throughout operation was not significantly linked to a higher TAG
accumulation.