A solvatofluorochromic silicon-substituted xanthene dye useful in bioimaging
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Espinar Barranco, Laura; Luque Navarro, Pilar María; Strnad, Maja Ana; Herrero-Foncubierta, Pilar; Crovetto González, Luis; Miguel Álvarez, Delia; Girón González, María Dolores; Orte Gutiérrez, Ángel; Cuerva Carvajal, Juan Manuel; Salto González, Rafael; Alvarez-Pez, José María; Paredes Martínez, José ManuelMateria
Hetero-xanthene dyes Solvatofluorochromism Fluorescent dyes Biosensors Photophysics
Date
2019-09Referencia bibliográfica
Laura Espinar-Barranco, Pilar Luque-Navarro, Maja A. Strnad, Pilar Herrero-Foncubierta, Luis Crovetto, Delia Miguel, Maria D. Giron, Angel Orte, Juan M. Cuerva, Rafael Salto, Jose M. Alvarez-Pez, Jose M. Paredes, A solvatofluorochromic silicon-substituted xanthene dye useful in bioimaging, Dyes and Pigments, Volume 168, 2019, Pages 264-272, ISSN 0143-7208, https://doi.org/10.1016/j.dyepig.2019.04.024.
Sponsorship
This work was funded by grants CTQ2017-85658-R, CTQ2014-55474-C2-2-R (Spanish Ministry of Economy and Competitiveness; Agencia Estatal de Investigacion, AEI; and European Regional Development Fund, ERDF), QM2012-790 (Junta de Andalucía), and a grant from the Fundación Botín.Abstract
In this work, we have performed an in-depth study of the photophysics and solvatofluorochromism of a red-emitting Si-xanthene dye, an analog of Tokyo Magenta (TM) historically developed by Egawa et al. (Chem. Commun. 2011, 47, 4162–4164). The results show a strong dependency of the emission properties of 2-Me-4-OMe-TM on the polarity of the solvent. For instance, the dye exhibited an increase in its fluorescence lifetime with the decrease in solvent polarity. Therefore, in this work, this spectral behavior has been used as a new approach for determining the intracellular microenvironment polarity through the measurement of its fluorescence lifetime by Fluorescence Lifetime Imaging Microscopy (FLIM). Our experiments confirmed the ability of the dye to detect changes in polarity between different intracellular compartments.