Selecting FRET pairs for visualizing amyloid aggregation

Ruiz Arias, Álvaro; Jurado Palomares, Rocío; Fueyo Gonzalez, Francisco; Herranz, Rosario; Gálvez Rodríguez, Natividad; González Vera, Juan Antonio; Orte Gutiérrez, Ángel

doi:10.1016/j.rechem.2021.100275

1-s2.0-S2211715621001806-main.pdf (1.907Mb)

Identificadores

URI: http://hdl.handle.net/10481/72590

DOI: 10.1016/j.rechem.2021.100275

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Editorial

Elsevier

Materia

Amyloid fibrils

Protein aggregation

Solvatochromic dyes

Biomarkers

FLIM

STED microscopy

Date

2021-12-30

Referencia bibliográfica

A. Ruiz-Arias et al. Selecting FRET pairs for visualizing amyloid aggregation. Results in Chemistry 4 (2022) 100275 [https://doi.org/10.1016/j.rechem.2021.100275]

Sponsorship

Spanish Ministerio de Educación y Formación; Grant CTQ2017–85658-R funded by MCIN/AEI/10.13039/501100011033/FEDER “Una manera de hacer Europa”; Grants PID2019–104366RB-C22 and PID2020–114256RBI00 funded by MCIN/AEI/10.13039/ 501100011033

Abstract

In a recent work, we reported a methodology for imaging the different stages of amyloid aggregation in quantitative multiparametric dual-color fluorescence lifetime imaging (FLIM) and superresolution microscopy by using a pair of dyes capable of binding aggregates and undergoing subsequent intra-aggregate energy transfer (FRET) (RuizArias et al. Sensors Actuat. B, 2022, 350:130882). In this microarticle we present the optimization process for choosing the best pair of dyes through a screening of different naphthalimides and quinolimides and other known amyloid-binding dyes.

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