In Vitro Wound-Healing Properties of Water-Soluble Terpenoids Loaded on Halloysite Clay
Metadatos
Afficher la notice complèteAuteur
Marinelli, Lisa; Borrego Sánchez, Ana María; García Villén, Fátima; Viseras Iborra, César AntonioEditorial
MDPI
Materia
Clay Halloysite Skin regeneration Terpenoids Wound healing
Date
2021-07-22Referencia bibliográfica
Marinelli, L... [et al.]. In Vitro Wound-Healing Properties of Water-Soluble Terpenoids Loaded on Halloysite Clay. Pharmaceutics 2021, 13, 1117. [https://doi.org/10.3390/pharmaceutics13081117]
Patrocinador
Italian Ministry of Education, University and Research (University "G. d'Annunzio" of Chieti-Pescara) FAR 2020Résumé
Recently, mineral healing clays have gained much attention for wound-dressing applications.
Here, we selected halloysite (HAL) clay as a biocompatible, non-toxic material that is useful
as a drug delivery system to enhance the healing properties of water-soluble terpenoids 1-3 (T1-3).
Terpenoids-loaded HAL clay (TH1-3) was prepared and characterized by adsorption equilibrium
studies, X-ray powder diffraction (XRPD), thermogravimetric analysis (TGA), differential scanning
calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy, and release studies. The results
reveal that T1-3 were adsorbed at the HAL surface with good efficiency. The prevalent mechanism
of drug retention is due to the adsorption via electrostatic interactions between the cationic groups
of the T1-3 and the HAL’s external surface. Release studies demonstrated that T3 was released
in a higher percentage (>60%) compared to T1-2 ( 50%). Additionally, TH1-3 were assessed for
their antimicrobial activity and capability to promote the re-epithelialization of scratched HaCat
monolayers, through the time-kill test and the wound-healing assays, respectively. The results reveal
that all the tested formulations were able to reduce the microbial growth after 1 h of incubation and
that they ensured complete wound closure after 48 h. Furthermore, at the concentration of 1 g/mL,
TH3 exhibited 45% wound closure at 24 h, compared to TH1 (27%) and TH2 (30%), proving to be the
best candidate in making the tissue-repair process easier and faster.