Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem-Cell-Derived ECM for Cartilage Tissue Engineering
Metadatos
Mostrar el registro completo del ítemAutor
Antich Acedo, Cristina; Jiménez, Gema; Vicente Álvarez-Manzaneda, Juan De; López Ruiz, Elena; Chocarro Wrona, Carlos; Griñán Lisón, Carmen; Carrillo Delgado, Esmeralda Esperanza; Montañez, Elvira; Marchal Corrales, Juan AntonioEditorial
Wiley
Fecha
2021-03Referencia bibliográfica
Antich, C., JimÚnez, G., de Vicente, J., Lˇpez‐Ruiz, E., Chocarro‐Wrona, C., Gri˝ ßn‐Lisˇn, C., ... & Marchal, J. A. (2021). Development of a Biomimetic Hydrogel Based on Predifferentiated Mesenchymal Stem‐Cell‐Derived ECM for Cartilage Tissue Engineering. Advanced Healthcare Materials, 2001847. [DOI: 10.1002/adhm.202001847]
Patrocinador
Spanish Ministry of Education, Culture and Sports BOE-A-2014-13539; Ministerio de Economía, Industria y Competitividad (ERDF funds) RTC-2016-5451-1; Instituto de Salud Carlos III FMM-AP17196-2019; Consejería de Economía, Conocimiento, Empresas y Universidad de la Junta de Andalucía (ERDF funds) B-CTS-230-UGR18 PY18-2470 SOMM17-6109 P18-FR-2465; Instituto de Salud Carlos III, ERDF funds DTS19/00145Resumen
The use of decellularized extracellular matrix (dECM) as a biomaterial has
been an important step forward for the development of functional tissue
constructs. In addition to tissues and organs, cell cultures are gaining a lot of
attention as an alternative source of dECM. In this work, a novel biomimetic
hydrogel is developed based on dECM obtained from mesenchymal stem cells
(mdECM) for cartilage tissue engineering. To this end, cells are seeded under
specific culture conditions to generate an early chondrogenic extracellular
matrix (ECM) providing cues and elements necessary for cartilage
development. The composition is determined by quantitative, histological,
and mass spectrometry techniques. Moreover, the decellularization process is
evaluated by measuring the DNA content and compositional analyses, and
the hydrogel is formulated at different concentrations (3% and 6% w/v).
Results show that mdECM derived hydrogels possess excellent
biocompatibility and suitable physicochemical and mechanical properties for
their injectability. Furthermore, it is evidenced that this hydrogel is able to
induce chondrogenesis of mesenchymal stem cells (MSCs) without
supplemental factors and, furthermore, to form hyaline cartilage-like tissue
after in vivo implantation. These findings demonstrate for the first time the
potential of this hydrogel based on mdECM for applications in cartilage repair
and regeneration.