Caloric restriction, physical exercise, and CB1 receptor blockade as an efficient combined strategy for bodyweight control and cardiometabolic status improvement in male rats
Metadatos
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López Trinidad, Luisa M.; Martínez Martínez, Rosario; Kapravelou, Garyfallia; Aranda Ramírez, Pilar; Porres Foulquie, Jesús María; López-Jurado Romero De La Cruz, MaríaEditorial
Nature Research
Fecha
2021-02-19Referencia bibliográfica
Trinidad, L. M. L., Martinez, R., Kapravelou, G., Galisteo, M., Aranda, P., Porres, J. M., & Lopez-Jurado, M. (2021). Caloric restriction, physical exercise, and CB1 receptor blockade as an efficient combined strategy for bodyweight control and cardiometabolic status improvement in male rats. Scientific Reports, 11(1), 1-16. [https://doi.org/10.1038/s41598-021-83709-9]
Patrocinador
Spanish Ministry of Science, Innovation and Universities; European Commission DEP2014-58296-R RTC-2017-6540-1 RTI2018-100934-B-I00; European CommissionResumen
Obesity is critically associated with the development of insulin resistance and related cardiovascular
and kidney diseases. Several strategies for weight loss have been developed but most of them exhibit
a post-intervention rebound effect. Here, we aimed to design combined weight-loss strategies
of caloric restriction, physical exercise, and administration of a CB1 receptor blocker to inhibit
food intake that also accomplish the objectives of lost-weight maintenance and improvement of
cardiovascular and renal function. Diet-induced obesity (DIO) was generated in Sprague Dawley
rats for 12 weeks to test the effects of single or combined strategies (i.e. caloric restriction, mixed
training protocol, and/or administration of appetite suppressant) on caloric intake, body weight,
cardiovascular and renal functionality resulting from a weight-loss intervention period of 3 weeks
followed by 6 weeks of weight maintenance. Consumption of a high-fat diet (HFD) caused a significant
increase in body weight (5th week of the experimental period) and led to the development of insulin
resistance, cardiovascular, and renal alterations. The different interventions tested, resulted in a
significant body weight loss and improved glucose metabolism, aerobic capacity, electrocardiographic
parameters, vascular expression of adhesion molecules and inflammatory mediators, and renal
functionality, reaching values similar to the control normocaloric group or even improving them.
Successful maintenance of lost weight was achieved along a 6-week maintenance period in addition to
adequate health status. In conclusion, the weight-loss and maintenance intervention strategies tested
were efficient at reversing the obesity-related alterations in body weight, glucose metabolism, aerobic
capacity, cardiovascular and renal functionality. The beneficial action was very consistent for caloric
restriction and physical exercise, whereas administration of a CB1 receptor blocker complemented
the effects of the prior interventions in some parameters like body weight or aerobic capacity, and
showed specific actions in renal status, increasing glomerular filtration rate and diuresis. Overall, the
novelty of our study relies on the easy implementation of combined strategies for effective weight
management that resulted in significant health benefits.