Apoferritin Amyloid-Fibril Directed the In Situ Assembly and/or Synthesis of Optical and Magnetic Nanoparticles
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Materia
Amyloid fibrils Apoferritin protein B -lactoglobulin Magnetic nanoparticle Gold nanoparticles MRI and optical imaging Bionanomaterials
Date
2021-01-08Referencia bibliográfica
Jurado, R.; Gálvez, N. Apoferritin Amyloid-Fibril Directed the In Situ Assembly and/or Synthesis of Optical and Magnetic Nanoparticles. Nanomaterials 2021, 11, 146. [https://doi.org/10.3390/nano11010146]
Sponsorship
Junta de Andalucía P18-FQM-1373; MINECO Project PID2019-111461GB-100Abstract
The coupling of proteins that can assemble, recognise or mineralise specific inorganic
species is a promising strategy for the synthesis of nanoscale materials with a controllable morphology
and functionality. Herein, we report that apoferritin protein amyloid fibrils (APO) have the ability
to assemble and/or synthesise various metal and metal compound nanoparticles (NPs). As such,
we prepared metal NP–protein hybrid bioconjugates with improved optical and magnetic properties
by coupling diverse gold (AuNPs) and magnetic iron oxide nanoparticles (MNPs) to apoferritin
amyloid fibrils and compared them to the well-known -lactoglobulin (BLG) protein. In a second
approach, we used of solvent-exposed metal-binding residues in APO amyloid fibrils as nanoreactors
for the in situ synthesis of gold, silver (AgNPs) and palladium nanoparticles (PdNPs). Our results
demonstrate, the versatile nature of the APO biotemplate and its high potential for preparing
functional hybrid bionanomaterials. Specifically, the use of apoferritin fibrils as vectors to integrate
magnetic MNPs or AuNPs is a promising synthetic strategy for the preparation of specific contrast
agents for early in vivo detection using various bioimaging techniques.