Menstrual blood‑derived stromal cells modulate functional properties of mouse and human macrophages
Metadata
Show full item recordAuthor
Martínez Aguilar, Rocío; Romero Pinedo, Salvador; Ruiz Magaña, María José; García Olivares, Enrique Fernando; Ruiz Ruiz, María Carmen; Abadía Molina, Ana ClaraEditorial
Nature Research
Date
2020-12-07Referencia bibliográfica
Martínez-Aguilar, R., Romero-Pinedo, S., Ruiz-Magaña, M. J., Olivares, E. G., Ruiz-Ruiz, C., & Abadía-Molina, A. C. (2020). Menstrual blood-derived stromal cells modulate functional properties of mouse and human macrophages. Scientific reports, 10(1), 1-14. [| https://doi.org/10.1038/s41598-020-78423-x]
Sponsorship
Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016, ISCIII-Subdirección General de Evaluación y Fomento de la Investigación, Ministerio de Economía y Competitividad, Spain PI16/01642 PI10/01096; European Union (EU); Catedra de Investigación Antonio Chamorro-Alejandro Otero, Universidad de Granada CACH2017-1Abstract
Menstrual blood-derived stromal cells (MenSCs) are emerging as a strong candidate for cell-based
therapies due to their immunomodulatory properties. However, their direct impact on innate immune
populations remains elusive. Since macrophages play a key role in the onset and development of
inflammation, understanding MenSCs implication in the functional properties of these cells is required
to refine their clinical effects during the treatment of inflammatory disorders. In this study, we
assessed the effects that MenSCs had on the recruitment of macrophages and other innate immune
cells in two mouse models of acute inflammation, a thioglycollate (TGC)-elicited peritonitis model
and a monobacterial sepsis model. We found that, in the TGC model, MenSCs injection reduced the
percentage of macrophages recruited to the peritoneum and promoted the generation of peritoneal
immune cell aggregates. In the sepsis model, MenSCs exacerbated infection by diminishing the
recruitment of macrophages and neutrophils to the site of infection and inducing defective bacterial
clearance. Additional in vitro studies confirmed that co-culture with MenSCs impaired macrophage
bactericidal properties, affecting bacterial killing and the production of reactive oxygen intermediates.
Our findings suggest that MenSCs modulate the macrophage population and that this modulation
must be taken into consideration when it comes to future clinical applications.