Interactions between Primary Neurons and Graphene Films with Different Structure and Electrical Conductivity
Metadatos
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Wiley
Materia
2D materials Electrical conductivity Hydrophilic Neuronal networks Poly(ethylene terephthalate)
Date
2020-09-13Referencia bibliográfica
Capasso, A., Rodrigues, J., Moschetta, M., Buonocore, F., Faggio, G., Messina, G., ... & Bramini, M. (2020). Interactions between Primary Neurons and Graphene Films with Different Structure and Electrical Conductivity. Advanced Functional Materials, 2005300. [DOI: 10.1002/adfm.202005300]
Patrocinador
European Union (EU) 785219-Graphene Flagship-Core2; Ministero degli Affari Esteri e Cooperazione Internazionale of Italy (Farnesina-MAECI) MAE0057294; Basic Science Research Program; Creative Materials Discovery Program; International Research & Development Program through the NRF of Korea 2016M3A7B4910940 2018M3D1A1058793 2019K1A3A1A25000267; European Union's Horizon 2020 under the Marie Skodowska-Curie Action-COFUND Athenea3i grant 754446; European Union's Horizon 2020 research and innovation program under the Marie Skodowska-Curie grant 713640Résumé
Graphene-based materials represent a useful tool for the realization of novel
neural interfaces. Several studies have demonstrated the biocompatibility of
graphene-based supports, but the biological interactions between graphene and
neurons still pose open questions. In this work, the influence of graphene films
with different characteristics on the growth and maturation of primary cortical
neurons is investigated. Graphene films are grown by chemical vapor deposition
progressively lowering the temperature range from 1070 to 650 °C to change the
lattice structure and corresponding electrical conductivity. Two graphene-based
films with different electrical properties are selected and used as substrate for
growing primary cortical neurons: i) highly crystalline and conductive (grown
at 1070 °C) and ii) highly disordered and 140-times less conductive (grown at
790 °C). Electron and fluorescence microscopy imaging reveal an excellent
neuronal viability and the development of a mature, structured, and excitable
network onto both substrates, regardless of their microstructure and electrical
conductivity. The results underline that high electrical conductivity by itself is
not fundamental for graphene-based neuronal interfaces, while other physico–
chemical characteristics, including the atomic structure, should be also considered
in the design of functional, bio-friendly templates. This finding widens the
spectrum of carbon-based materials suitable for neuroscience applications.