A Box-Behnken Design for Optimal Green Extraction of Compounds from Olive Leaves That Potentially Activate the AMPK Pathway
Metadatos
Afficher la notice complèteAuteur
Martín-García, Beatriz; Pimentel Moral, Sandra; Gómez Caravaca, Ana María; Arráez Román, David; Segura Carretero, AntonioEditorial
MDPI
Materia
Microwave assisted extraction Extract of olive leaves HPLC-ESI-TOF-MS AMPK Box-Behnken Design
Date
2020-07-03Referencia bibliográfica
Martín-García, B., Pimentel-Moral, S., Gómez-Caravaca, A. M., Arráez-Román, D., & Segura-Carretero, A. (2020). A Box-Behnken Design for Optimal Green Extraction of Compounds from Olive Leaves That Potentially Activate the AMPK Pathway. Applied Sciences, 10(13), 4620. [doi: 10.3390/app10134620]
Patrocinador
Spanish Ministry of Economy and Competitiveness (MINECO) AGL2015-67995-C3-2; Ministry of Science, Innovation and Universities RTI2018-096724-B-C22; Ministry of Economy, Knowledge, Business and University of the Junta de Andalucia B-AGR-466-UGR18Résumé
Olive leaves contain bioactive compounds that have been shown to activate AMP-activated
protein kinase (AMPK), which decreases intracellular lipid accumulation. Microwave-assisted
extraction (MAE) is a green extraction technique that is frequently used in the recovery of phenolic
compounds from plants. Thus, in this study, a Box-Behnken design was used to optimize MAE
conditions such as temperature, percentage of ethanol and extraction time to obtain the maximum
content of total compounds and compounds that activate AMPK. To this end, all extracts were
characterized by High-Performance Liquid Chromatography Coupled to Electrospray Ionization
Time-of-Flight Mass Spectrometry (HPLC-ESI-TOF-MS). The optimum conditions to obtain the
highest content of total compounds were 123 ◦C, 100% of ethanol/water (v/v) and 23 min, whereas the
optimum conditions for the highest amount of compounds that activate AMPK were 111 ◦C, 42% of
ethanol/water (v/v) and 23 min. Thus, a multi-analysis by desirability was carried out to establish
MAE optimal conditions for both responses. The optimum conditions were 111 ◦C, 100% EtOH and
23 min with a desirability of 0.97, which means that the responses are close to their individual optimal
values. As a result, the olive leaf extract obtained at these optimal MAE conditions has great potential
to be effective in the treatment of obesity.